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羊胆汁中甘氨胆酸提取工艺研究
引用本文:李慧峰,李培锋,赵珍.羊胆汁中甘氨胆酸提取工艺研究[J].安徽农业科学,2008,36(20).
作者姓名:李慧峰  李培锋  赵珍
作者单位:1. 内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特,010018;河北北方学院动物科技学院,河北,张家口,075000
2. 内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特,010018
3. 内蒙古医学院,内蒙古呼和浩特,010018
摘    要:目的]开展从羊胆汁中提取、分离和纯化甘氨胆酸的工艺研究。方法]采用除蛋白、除色素、回收乙醇、盐析、萃取等生物化学方法,从羊胆汁中分离提取甘氨胆酸。采用了吸附色谱法和薄层层析色谱法分离纯化和鉴定了甘氨胆酸。结果]结果表明,该研究方法所提取的GCA纯度达97.3%。结论]首次建立了从羊胆汁中提取甘氨胆酸的技术路线,为临床用药的进一步开发奠定了基础。

关 键 词:羊胆汁  甘氨胆酸  提取

Study on Extraction Techniques of Glycocholic Acid from Sheep Bile
LI Hui-feng et al.Study on Extraction Techniques of Glycocholic Acid from Sheep Bile[J].Journal of Anhui Agricultural Sciences,2008,36(20).
Authors:LI Hui-feng
Abstract:Objective] The research aimed to study the extraction techniques of Glycocholic acid(GCA) from sheep bile.Method] GCA from sheep bile was obtained through deproteinization,depigmentation,ethanol reclamation,salting-out,extraction and anhydration.Adsorption chromatography and thin-layer chromatography was respectively used in disassociation and identification of GCA.Result] The purity of GCA reached to 97.3%.Conclusion] Extraction techniques of GCA from sheep bile was set up and established the base for the development of clinical medicine.
Keywords:Sheep bile  Glycocholic acid  Extraction
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