Identification of QTL underlying the resistance of soybean to pod borer, <Emphasis Type="Italic">Leguminivora glycinivorella</Emphasis> (Mats.) obraztsov,and correlations with plant,pod and seed traits

Soybean (Glycine max L. Merr.) pod borer (Leguminivora glycinivorella (Mats.) Obraztsov) (SPB) results in severe loss in soybean yield and quality in certain regions of the world, especially in Northeastern China, Japan and Russia. The aim here was to evaluate the inheritance of pod borer resistance and to identify quantitative trait loci (QTL) underlying SPB resistance for the acceleration of the control of this pest. Used were the 129 recombinant inbred lines (RILs) of the F_5:6 derived population from ‘Dong Nong 1068’ × ‘Dong Nong 8004’ and 131 SSR markers. Correlations between the percentage of damaged seeds (PDS) by pod borer and plant, pod and seed traits that were potentially related to SPB resistance were analyzed. The results showed highly significant correlations between PDS by pod borer and plant height (PH), maturity date (MA), pod color (PC), pubescence density (PB), 100-seed weight (SW) and protein content existed. Soybeans with dwarf stem, light color of pod coat, small seeds, lower density of pubescence, early maturity and low content of protein seemed to have higher resistance to SPB. The correlated traits had potential to inhibit egg deposition and thereby to decrease the damage by SPB. Three QTL directly associated with the resistance to SPB judged by PDS at harvest were identified. qRspb-1 (Satt541–Satt253) and qRspb-2 (Satt253–Satt314) were both on linkage group (LG) H and qRspb-3 (Satt288–Satt199) on LG G. The three QTL explained 10.96, 9.73 and 11.59% of the phenotypic variation for PDS, respectively. In addition, 12 QTL that underlay 10 of 13 traits potentially related with SPB resistance were found. These QTL detected jointly provide potential for marker assisted selection to improve cultivar resistance to SPB. Guiyun Zhao, Jian Wang, and Yingpeng Han have equal contribution to the paper.