不同植物生长调节物质处理对吴茱萸组织培养的影响

以吴茱萸Euodia rutaecarpa雌株再生苗叶片为材料，研究了不同植物生长调节物质处理对其组织培养过程的影响。结果表明：添加1.0 mg·L^－16-苄氨基腺嘌呤（6-BA） 和1.0 mg·L^－1 6-BA ＋ 0.1 mg·L^－1 α-萘乙酸（NAA）等2种处理均可诱导叶片叶柄端形成愈合组织，随后分化出不定芽。而其余处理只能诱导愈合组织而未见不定芽的分化，其中以添加2，4-二氯苯氧乙酸（2，4-D）诱导的愈合组织最佳。单独添加NAA不能诱导不定芽分化，但与6-BA同时添加时可增强6-BA的诱导效果；随着6-BA质量浓度的提高，不定芽分化率表现出先升高后下降的趋势；类似的现象也发生在不定芽的增殖和壮苗过程中，适当质量浓度的6-BA有助于增殖和壮苗，但质量浓度过高容易导致玻璃化。随着NAA质量浓度的升高，吴茱萸再生植株生根速度加快，根变粗，侧根增多，生根率和移栽成活率均提高，但NAA质量浓度过高不利于侧根生长和移栽。获得的吴茱萸再生植株移栽到大田1.5 a后即可成熟开花。图1表4参10

Plant growth regulator treatments for a tissue culture of Euodia rutaecarpa

To determine the effects of different plant growth regulator treatments,including 6-benzylaminopurine（6-BA）,1-naphthlcetic acid（NAA）,2,4-dichlorophenoxy acetic acid（2,4-D）,and N-phenyl-N＇-1,2, 3-thidiazol-5-yl-urea（TDZ）,on various processes of tissue culture in female plants of Euodia rutaecarpa（Juss.） Benth,leaves of regenerated shoots were used.Results showed that 1.0 mg·L-1 6-BA alone or with 0.1 mg·L-1 NAA induced both callus and adventitious buds.The other plant growth regulator treatments induced the formation of callus,but failed to induce adventitious buds.NAA by itself did not induce adventitious buds,but it did promote the effects of 6-BA.As the concentration of 6-BA increased,the rate of bud differentiation first increased and then declined,a similar trend happened with 6-BA on morphogenesis of adventitious buds.With an optimal concentration,6-BA promoted proliferation,whereas a high concentration led to vitrification.Also,0-0.1 mg·L-1 NAA promoted rooting and roots appeared faster and stouter as NAA increased.Moreover,the survival rate after transplanting increased.However,0.5 mg·L-1 NAA inhibited the growth of lateral roots and the transplanting procedure.After transplanting in the field for 1.5 years,the regenerated plants bloomed.[Ch,1 fig.4 tab.10 ref.]