| 枇杷叶片和果实总蛋白质提取及双向电泳的优化方法 |
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| 引用本文： | 马斌,孙骏威,余初浪,徐红霞,李素芳,陈贤.枇杷叶片和果实总蛋白质提取及双向电泳的优化方法[J].果树学报,2011(2):358-362. |
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| 作者姓名： | 马斌 孙骏威 余初浪 徐红霞 李素芳 陈贤 |
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| 作者单位： | 中国计量学院生命科学学院;浙江省生物计量及检验检疫技术重点实验室;浙江省农业科学院病毒学与生物技术研究所; |
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| 基金项目： | 浙江省自然科学基金(Y3080166,Y307577) |
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| 摘 要： | 以枇杷叶片和果实为材料,探索适于枇杷总蛋白质分析的双向电泳方法.比较2种不同蛋白质提取方法对双向电泳结果的影响,并对双向电泳蛋白质上样量、等电聚焦条件等进行了优化,探索出一种适于枇杷叶片和果实总蛋白质分析的高分辨率、高重复性双向电泳方法.采用酚抽法提取枇杷叶片和果实蛋白质,以24 cm、pH 4~7的IPG胶条,按15...
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| 关 键 词： | 枇杷 蛋白质 双向电泳 |
Optimization of protein isolation and method of two-dimensional electrophoresis from leaves and fruit of Loquat(Eriobotrya japonica) |
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| MA Bin,SUN Jun-wei,YU Chu-lang,XU Hong-xia,LI Su-fang,CHEN Xian.Optimization of protein isolation and method of two-dimensional electrophoresis from leaves and fruit of Loquat(Eriobotrya japonica)[J].Journal of Fruit Science,2011(2):358-362. |
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| Authors: | MA Bin SUN Jun-wei YU Chu-lang XU Hong-xia LI Su-fang CHEN Xian |
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| Institution: | MA Bin1,3,SUN Jun-wei1,YU Chu-lang2,XU Hong-xia2,LI Su-fang1,CHEN Xian2(1College of Life Sciences,China Ji Liang University,Hangzhou,Zhejiang 310018 China,2Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Zhejiang 310021 China,3Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine,Zhejiang 310018 China) |
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| Abstract: | A better extraction method was established to analyze woody plant protein proteolysis and two-dimensional gel electrophoresis by using Loquat(Eriobotrya japonica) leaves and fruits as experiment materials.Two total protein extraction methods,including trichloroacetic acid/acetone precipitation method and phenol extraction method were compared.We optimized protein sample size and isoelectric focusing section,and designed a good repeatability and high-resolution two dimensional electrophoresis technology to a... |
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| Keywords: | Loquat(Eriobotrya japonica) Protein Two-dimensional electrophoresis |
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