| 甜瓜抗蔓枯病基因Gsb-2的ISSR分子标记 |
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| 引用本文： | 张永兵,陈劲枫,伊鸿平,钱春桃,吴明珠.甜瓜抗蔓枯病基因Gsb-2的ISSR分子标记[J].果树学报,2011(2):296-300. |
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| 作者姓名： | 张永兵 陈劲枫 伊鸿平 钱春桃 吴明珠 |
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| 作者单位： | 作物遗传与种质创新国家重点实验室·南京农业大学园艺学院;新疆农业科学院哈密瓜研究中心;海南省甜瓜西瓜遗传育种重点实验室; |
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| 基金项目： | 新疆自治区自然科学基金项目(200821174); 新疆自治区高技术研究发展计划项目(200811107); 新疆自治区科研院所改革与发展专项资金项目(2009022) |
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| 摘 要： | 甜瓜蔓枯病(Didymella bryoniae)是危害我国甜瓜的一种主要病害.以抗蔓枯病种质资源PI 157082与感病的甜瓜自交系白皮脆为亲本,建立了抗感F2代群体,对亲本、F1及134个F2代群体进行了苗期抗蔓枯病接种鉴定.利用集团分离分析法(bulked segregant analysis,BSA)在F2代建...
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| 关 键 词： | 甜瓜 蔓枯病 ISSR标记 |
ISSR marker linked to gene Gsb-2 resistant to gummy stem blight in melon(Cucumis melo) |
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| ZHANG Yong-bing,CHEN Jin-feng,YI Hong-ping,QIAN Chun-tao,WU Ming-zhu.ISSR marker linked to gene Gsb-2 resistant to gummy stem blight in melon(Cucumis melo)[J].Journal of Fruit Science,2011(2):296-300. |
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| Authors: | ZHANG Yong-bing CHEN Jin-feng YI Hong-ping QIAN Chun-tao WU Ming-zhu |
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| Institution: | ZHANG Yong-bing1,2,3,CHEN Jin-feng1,YI Hong-ping2,QIAN Chun-tao1,WU Ming-zhu2,3(1College of Horticulture,State Key Laboratory of Crop Genetics and Germplasm Enhancemen,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China,2Center of Hami Melon,Xinjiang Academy of Agricultural Sciences,Urumqi,Xinjiang 830091 China,3Hainan Province Key Laboratory of Melon-Watermelon Genetics and Breeding,Sanya,Hainan 512074 China) |
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| Abstract: | Gummy stem blight(GSB) caused by fungus Didymella bryoniae(Auersw.) Rehm is one of the major diseases of melon,and causes severe losses.Parents,F1 and F2 population derived from the cross of the resistant germplasm PI 157082 and susceptible inbred line Baipicui were identified for their resistance to GSB in seedling stage.Using bulked segregant analysis(BSA),72 ISSR primers were screened between resistant and susceptible bulks.A 560 bp fragment specifically amplified with ISSR-57 primer was present in the r... |
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| Keywords: | Melon Gummy stem blight ISSR marker |
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