| 资阳香橙×枳杂种苗鉴定及耐碱株系筛选 |
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| 引用本文： | 曹立,彭良志,彭爱红,凌丽俐,江东,王福生,范达,王军政,马小焕,张雯雯,常青华,王小雪. 资阳香橙×枳杂种苗鉴定及耐碱株系筛选[J]. 果树学报, 2011, 0(1) |
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| 作者姓名： | 曹立 彭良志 彭爱红 凌丽俐 江东 王福生 范达 王军政 马小焕 张雯雯 常青华 王小雪 |
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| 作者单位： | 西南大学园艺园林学院;西南大学柑橘研究所;西南大学资源环境学院; |
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| 基金项目： | 国家科技支撑计划项目(2007BAD47B042007BAD61B05); 公益性行业(农业)科研专项(nyhyzx07-023); 国家现代农业(柑橘)产业技术体系建设专项; 重庆市科委重大专项(CSTC2007AA1016); 重庆市自然科学基金(CSTC2009BB1137) |
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| 摘 要： | 为培育耐碱的杂种砧木,以耐碱品种资阳香橙(Citrus junos Sieb.ex Tanaka)与不耐碱的枳[Poncirus trifoliata(L.)Raf.]杂交,将杂种F1群体经形态学与SSR鉴定杂种真实性,并通过叶片黄化程度分级、叶绿素与有效铁质量分数的测定以筛选耐碱杂种单株。结果表明,(1)与母本资阳香橙叶片形态上无明显差异的F1都是珠心苗。(2)真实杂种苗从第10片叶开始都有三出复叶。(3)筛选到2对SSR引物能鉴定出全部杂种苗。(4)在碱性土上,耐碱杂种苗叶绿素cha+b的质量分数显著低于耐碱亲本资阳香橙,而极显著高于不耐碱亲本枳;但叶绿素a的质量分数显著高于父母本。5、耐碱杂种叶片中有效铁质量分数显著高于枳。研究获得了2株耐碱能力较强的真实杂种苗。
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| 关 键 词： | 柑橘 砧木 杂种鉴定 耐碱 SSR鉴定 |
Preliminary identification of alkali-resistance hybrid seedlings of Junos and Trifoliate orange |
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| CAO Li,PENG Liang-zhi,PENG Ai-hong,LING Li-li,JIANG Dong,,WANG Fu-sheng,FAN Da,WANG Jun-zheng,MA Xiao-huan,ZHANG Wen-wen,CHANG Qing-hua,WANG Xiao-xue. Preliminary identification of alkali-resistance hybrid seedlings of Junos and Trifoliate orange[J]. Journal of Fruit Science, 2011, 0(1) |
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| Authors: | CAO Li PENG Liang-zhi PENG Ai-hong LING Li-li JIANG Dong WANG Fu-sheng FAN Da WANG Jun-zheng MA Xiao-huan ZHANG Wen-wen CHANG Qing-hua WANG Xiao-xue |
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| Affiliation: | CAO Li1,2,PENG Liang-zhi2,PENG Ai-hong2,LING Li-li2,JIANG Dong1,2*,WANG Fu-sheng1,FAN Da1,WANG Jun-zheng1,MA Xiao-huan1,ZHANG Wen-wen1,CHANG Qing-hua1,WANG Xiao-xue3(1College of Horticulture and Landscape,Southwest University,Chongqing 400715 China,Citrus Research institute,Chongqing 400712 Chian,3College of Resources and Environment,Chongqing 400715 Chian) |
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| Abstract: | In order to obtain high tolerance rootstocks to calcareous soil from citrus inter-genera offspring,hybridization were made with artificial cross-pollination method between Ziyangxiangcheng(Citrus junos Sieb.ex Tanaka) and Trifoliate orange [Poncirus trifoliata(L.) Raf.].The zygotic offspring were identified both by visible morphological characteristics and SSR markers.After growing on calcareous soil for one year,the tolerance capability of zygotic offspring was evaluated based on the degree of leaf yellowi... |
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| Keywords: | Citrus Rootstock Hybrid identification Alkali tolerance SSR |
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