| 小麦SSR连锁图谱的构建及多态性研究 |
| |
| 引用本文： | 石培春,王光利,张薇,曹连莆.小麦SSR连锁图谱的构建及多态性研究[J].新疆农业科学,2007,44(Z3):71-76. |
| |
| 作者姓名： | 石培春 王光利 张薇 曹连莆 |
| |
| 作者单位： | 1. 石河子大学农学院,新疆,石河子,832003;新疆兵团绿洲生态农业重点实验室,新疆,石河子,832003
2. 石河子大学农学院,新疆,石河子,832003 |
| |
| 摘 要： | 为构建小麦遗传图谱并对小麦主要蛋白品质性状进行QTL定位,以普通小麦99G44×京771的178个重组自交系RIL(F2:8)为作图群体,利用721对SSR引物筛选出RIL群体双亲表现多态性的引物180对,多态性频率为24.9%。利用这180对引物对RIL群体进行分析,共检测到98个多态性标记位点,利用Mapmaker 3.0 Mapdraw v2.1软件将其中的82个SSR位点绘在小麦21条染色体上。该图谱覆盖小麦基因组全长1532.38cM,标记间的平均遗传距离为19.15 cM。
|
| 关 键 词： | 小麦RIL群体 SSR标记 连锁图谱 多态性 |
| 文章编号： | 1001-4330(2007)S3-0071-06 |
| 修稿时间： | 2007年10月24 |
Construction of Wheat SSR Molecular Linkage Map and Its Polymorphism |
| |
| SHI Pei-chun,WANG Guang-li,ZHANG Wei,CAO Lian-pu.Construction of Wheat SSR Molecular Linkage Map and Its Polymorphism[J].Xinjiang Agricultural Sciences,2007,44(Z3):71-76. |
| |
| Authors: | SHI Pei-chun WANG Guang-li ZHANG Wei CAO Lian-pu |
| |
| Abstract: | 178 recombinant inbred llines(RILs) derived from 99G44×Jing 771 were used as mapping population in wheat.721 pairs of SSR primers were used to detect the polymorphisms between parents 99G44 and Jing 771.180 pairs of SSR primers with the polymorphisms between parents 99G44 and Jing 771 were selected to analyze RIL population.98 markers were detected by the primers mengtioned above .Only 82 markers were used to construct the molecular genetic Lingage map,and mapped on 21 chromosomes by Mapmaker 3.0 and Mapdraw v2.1.The map spaned about 1 532.38 cM in wheat genome.The average distance of markers was 19.15 cM. |
| |
| Keywords: | wheat RIL population SSR marker linkage map polymorphism |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
