|
|||||
|
|
| 野生罂粟BBE基因克隆及RNAi载体构建 | |
| 引用本文： | 梁倩倩,张金文,魏玉杰,陈志国,贾晓霞. 野生罂粟BBE基因克隆及RNAi载体构建[J]. 甘肃农业大学学报, 2009, 44(6) |
| 作者姓名： | 梁倩倩 张金文 魏玉杰 陈志国 贾晓霞 |
| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070;河西学院生命科学与工程系,甘肃,张掖,734000 2. 甘肃农业大学农学院,甘肃,兰州,730070 3. 甘肃农垦农业研究所,甘肃,武威,733006 |
| 基金项目： | 教育部博士学科点研究基金，甘肃省卫生厅重点中医药科技项目，甘肃省卫生厅重点中医药科技项目 |
| 摘 要： | 以野生罂粟(Papaver somniferum)试管苗幼叶为材料,用Trizol试剂盆提取总RNA,通过RT-PCR法获得BBE基因的cDNA片段,序列分析表明,所获得的cDNA序列全长1 608 bp,具有完整的开放阅读框(ORF),编码536个氨基酸,经blast检索该片段与GenBank中的小檗碱桥酶基因BBE(AF025430)同源性为94.84%.以中间载体pHANNIBAL和植物表达载体pART27为基础,构建了CaMV-35S启动子驱动的含小檗碱桥酶基因片段反向重复序列的RNAi双元表达载体pARB,为培育低吗啡,高蒂巴因的罂粟新品系奠定了基础. |
| 关 键 词： | 罂粟 BBE基因 RNAi载体构建 克隆 |
Cloning of BBE gene of Papave somniferum and construction of its RNAi expression vector |
|
| LIANG Qian-qian,ZHANG Jin-wen,WEI Yu-jie,CHEN Zhi-guo,JIA Xiao-xia. Cloning of BBE gene of Papave somniferum and construction of its RNAi expression vector[J]. Journal of Gansu Agricultural University, 2009, 44(6) | |
| Authors: | LIANG Qian-qian ZHANG Jin-wen WEI Yu-jie CHEN Zhi-guo JIA Xiao-xia |
| Affiliation: | LIANG Qian-qian1,2,ZHANG Jin-wen1,WEI Yu-jie3,CHEN Zhi-guo3,JIA Xiao-xia1(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China,2.Department of life Science and Engineering,Hexi University,Zhangye 734000,3.Gansu Academy of Reclamation Agricultural Research,Wuwei 733006,) |
| Abstract: | Total RNA was extracted from young leaf of opium poppy(Papaver somniferum L) by using Trizol regent.The berberine bridge enzyme(BBE) gene was cloned by RT-PCR and its coding sequence was analyzed.The result indicated that the full-length cDNA was 1 608 bp and contained a 1 608 bp open reading frame(ORF) encoding 536 amino acids.The sequence analyzed with software Blast was highly homologous to the BBE(AF025430)gene with a 94.84 % identity to it.Then the promoter CaMV 35S driven,plant siRNA expression vector... |
| Keywords: | Papaver somniferum berberine bridge enzyme gene construction of RNAi plant expression vector clone |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |