取食不同寄主的草地贪夜蛾肠道微生物多样性分析

为绿色防控草地贪夜蛾Spodoptera frugiperda，基于16S rDNA和ITS高通量测序分析，对分别取食玉米、小麦以及田间常见杂草婆婆纳Veronica polita的草地贪夜蛾肠道细菌和真菌进行物种注释、多样性分析、物种组成分析及功能预测。结果显示，细菌注释共获得20门30纲72目106科164属214种，真菌注释共获得9门23纲46目90科154属237种。厚壁菌门、放线菌门和变形菌门为取食玉米、小麦和婆婆纳的草地贪夜蛾肠道优势细菌门，子囊菌门和担子菌门为取食玉米、小麦和婆婆纳的草地贪夜蛾肠道优势真菌门。肠球菌属Enterococcus为取食玉米、小麦和婆婆纳的草地贪夜蛾肠道细菌共同的优势属，相对丰度分别为70.86%、25.42%和72.02%；丝孢酵母属Tricho-sporon 和帚枝霉属 Sarocladium 为取食玉米的草地贪夜蛾肠道真菌的优势属，相对丰度分别为57.28%和17.38%，酵母菌目未分类属、小囊菌属Microascus和丝孢酵母属Trichosporon为取食小麦的草地贪夜蛾肠道真菌优势属，相对丰度分别为29.31%、27.01%和15.45%，双足囊菌科未分类属为取食婆婆纳的草地贪夜蛾肠道真菌优势属，相对丰度为61.79%。取食小麦的草地贪夜蛾肠道细菌群落的多样性最高，取食婆婆纳的草地贪夜蛾肠道真菌群落的多样性最高，取食婆婆纳的草地贪夜蛾肠道细菌和真菌群落的丰富度最高。取食玉米、小麦和婆婆纳的草地贪夜蛾肠道细菌群落功能主要与代谢通路相关，取食玉米、小麦和婆婆纳的草地贪夜蛾肠道真菌群落功能主要与基础代谢或生理功能相关。

Analysis of the diversity of intestinal microbiomes in fall armyworm Spodoptera frugiperda fed on different host plants

For sustainable control of fall armyworm Spodoptera frugiperda, based on 16S rDNA and ITS high-throughput sequencing analysis, species annotation, diversity analysis, species composition analysis and functional prediction of the gut bacteria and fungi of S. frugiperda feeding on maize, wheat and Veronica polita were carried out. The results showed that 214 species belonging to 164 genera, 106 families, 72 orders, 30 classes, 20 phyla were obtained by bacterial annotation, and 237 species belonging to 154 genera, 90 families, 46 orders, 23 classes, nine phyla were obtained by fungal annotation. Firmicutes, Actinobacteriota and Proteobacteria were found to be the dominant bacterial phyla in the intestines of S. frugiperda fed on maize, wheat and V. polita, and Ascomycota and Basidiomycota were the dominant fungi phyla in the intestines of S. frugiperda fed on maize, wheat and V. polita. Enterococcus was the dominant genus in gut bacteria of S. frugiperda feeding on maize, wheat and V. polita, with a relative abundance of 70.86%, 25.42% and 72.02%, respectively; Trichosporon and Sarocladium were the dominant genera of intestinal fungi of S. frugiperda feeding on maize, with a relative abundance of 57.28% and 17.38%, respectively. Unclassified Saccharomycetales, Microascus and Trichosporon were the dominant genera of intestinal fungi of S. frugiperda feeding on wheat, with a relative abundance of 29.31%, 27.01% and 15.45%, respectively. Unclassified genus in Dipodascaceae was the dominant genus of intestinal fungi in S. frugiperda feeding on V. polita, with a relative abundance of 61.79%. The diversity of gut bacterial communities of S. frugiperda feeding on wheat was the highest; the diversity of intestinal fungal community of S. frugiperda feeding on V. polita was the highest, and the richness of intestinal bacterial and fungal communities of S. frugiperda feeding on V. polita was the highest. The function of intestinal bacterial community in S. frugiperda feeding on maize, wheat and V. polita was mainly related to metabolic pathways. The function of intestinal fungal community of S. frugiperda feeding on maize, wheat and V. polita was mainly related to basal metabolism or physiological function.