Radiomutants of chrysanthemum (Dendranthema grandiflora Tzvelev) of the Lady group: RAPD analysis of the genetic diversity |
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| Authors: | J Lema-Rumiñska M Zalewska Z Sadoch |
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| Institution: | Department of Ornamental Plants and Vegetable Crops, University of Technology and Agriculture in Bydgoszcz, Bernardyńska 6, PL-85-029 Bydgoszcz, Poland, E-mail: lem-rum@atr.bydgoszcz.pl;Plant Breeding and Acclimatization Institute, Powstancow Wielkopolskich 10, PL-8 5-950 Bydgoszcz, Poland |
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| Abstract: | Random amplified polymorphic DNA (RAPD) markers were used to study the molecular characterization of 10 new radiomutants of chrysanthemum. The original cultivar ‘Richmond’ differed in genetic distance from its Lady group mutants. The analysis of genetic similarity indices revealed low diversity within the radiomutants. The dendrogram obtained after cluster analysis separated the new cultivars as a group that differed from the original cultivar ‘Richmond’. The Lady group cultivars, derived from one original cultivar by radiomutation, could be distinguished from each other by using RAPD markers of only a single primer or sets of two or three primers. Polymerase chain reaction analysis proved the efficiency of the RAPD method for DNA fingerprinting of the original cultivar ‘Richmond’ and its new radiomutants. |
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| Keywords: | Dendranthema grandiflora radiomutants RAPD DNA fingerprinting identification |
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