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黄瓜苗期猝倒病拮抗菌的鉴定
引用本文:楼兵干,张炳欣,Maarten Ryder,Stephen Barnett.黄瓜苗期猝倒病拮抗菌的鉴定[J].浙江大学学报(农业与生命科学版),2002,28(1):54-58.
作者姓名:楼兵干  张炳欣  Maarten Ryder  Stephen Barnett
作者单位:1. 浙江大学,植物保护系,浙江,杭州,310029
2. CSIRO Land and Water,Glen Osmond Southern Australia 5064
基金项目:澳大利亚国际农业发展中心资助项目 ( PN9680 ),国家自然科学基金资助项目 ( 3 0 0 70 5 11)
摘    要:通过甲基脂肪酸气相色谱分析法(GC-FAME)、Biolog细菌鉴定法(借助于计算机的数值分类法)和其它试验对黄瓜苗期猝倒病具有明显防治效果的16株拮抗菌株进行分类鉴定.结果表明:黄瓜苗期猝倒病拮抗菌的优势种为Pseudomonas aeruginosa 和 Bacillus brevis,其次为Stenotrophomonas maltophilia, B. polymyxa, Pseudomonas fluorescens 和 Agrobacterium radiobacter及一些还有待进一步鉴定的种.并对GC-FAME和 Biolog的鉴定结果进行了比较:在属的水平上两种方法鉴定一致的拮抗菌株为14株,占87.5%;在种的水平上鉴定一致的拮抗菌株为7株,占42.75%.GC-FAME和 Biolog对某些细菌的鉴定提供了快速而可靠的方法,但由于目前计算机数据库尚不完备,鉴定部分细菌如B. subtilis需用其它补充方法.

关 键 词:GC-FAME  Biolog  拮抗细菌鉴定
文章编号:1008-9209(2002)01-0054-05

Identification of bacteria antagonistic to cucumber seedling damping-off.
Maarten Ryder,Stephen Barnett.Identification of bacteria antagonistic to cucumber seedling damping-off.[J].Journal of Zhejiang University(Agriculture & Life Sciences),2002,28(1):54-58.
Authors:Maarten Ryder  Stephen Barnett
Abstract:Gas chromatographic analysis of fatty acid methyl esters (GC FAME),Biolog (computer aided numeric taxonomy method) and other tests were used for identification of 16 antagonistic bacteria against damping off of cucumber seedlings.The results from GC FAME,Biolog and other tests indicated that the dominant species were Pseudomonas aeruginosa and Bacillus brevis ,then Stenotrophomonas maltophilia,B.polymyxa,Pseudomonas fluorescens and Agrobacterium radiobacter and some other isolates showed antagonism which need to be identified.Comparison of the results on identification by both methods GC FAME and Biolog: 14 of 16 antagonistic bacteria showed the same at genus level obtained from two methods,the proportion about 87.5%;then 7 were in species, at 42.75%.GC FAME and Biolog provide rapid and reliable approaches for identification of some bacteria.However,complementary methods are needed for identification of some bacteria such as B.subtilis, due to insufficient database.
Keywords:GC  FAME  Biolog  Antagonistic bacterial identification
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