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蚕豆离体培养繁殖植株对高温及紫外线胁迫的反应
引用本文:陆瑞菊,屈巧玲,王亦菲,孙月芳,胡润田,康诗腾,许凌波,黄剑华. 蚕豆离体培养繁殖植株对高温及紫外线胁迫的反应[J]. 核农学报, 2006, 20(6): 469-472
作者姓名:陆瑞菊  屈巧玲  王亦菲  孙月芳  胡润田  康诗腾  许凌波  黄剑华
作者单位:1. 上海市农业科学院生物技术研究所,上海,201106;上海市农业遗传育种重点实验室,上海,201106
2. 上海市文来中学,上海,201101
摘    要:将蚕豆种子无菌苗(对照)和离体繁殖3代无菌苗作为供试材料,观察它们在高温和紫外线胁迫下的生长和根尖细胞有丝分裂的情况。试验结果表明,供试植株在高温和紫外线胁迫下在外观上表现叶片枯黄、植株死亡,在染色体水平上发生了畸变,紫外线胁迫造成的畸变程度大于高温胁迫,3代克隆苗的畸变程度大于种子苗。

关 键 词:蚕豆    快繁    高温和紫外线胁迫    染色体畸变
文章编号:1000-8551(2006)06-469-04
收稿时间:2006-02-15
修稿时间:2006-02-15

RESPONSE OF in vitro PROPAGATED PLANTLETS OF Vicia faba TO HIGH TEMPERATURE AND ULTRAVIOLET STRESS
LU Rui-ju,QU Qiao-ling,WANG Yi-fei,SUN Yue-fang,HU Run-tian,KANG Shi-teng,XU Ling-bo,HUANG Jian-hua. RESPONSE OF in vitro PROPAGATED PLANTLETS OF Vicia faba TO HIGH TEMPERATURE AND ULTRAVIOLET STRESS[J]. Acta Agriculturae Nucleatae Sinica, 2006, 20(6): 469-472
Authors:LU Rui-ju  QU Qiao-ling  WANG Yi-fei  SUN Yue-fang  HU Run-tian  KANG Shi-teng  XU Ling-bo  HUANG Jian-hua
Affiliation:1. Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106 ;2. Shanghai Key Lab. of Agricultural Genetics and Breeding, Shanghai 201106;3.Shanghai Wenlai Middle School. Shanghai 201101
Abstract:Using propagated-plantlets from the 3~(rd) generation of Vicia faba as materials,the effects of heat and ultraviolet stress on growth and the mitosis of root-tip cells of plantlets were studied.The results showed that the frequency of the plantlets with yellow leaf and dead plantlets and chromosomal aberrations were increased by use of both stress compared with CK,the effects of ultraviolet stress were stronger than that of heat stress.The frequency of chromosomal aberrations of plantlets from the 3~(rd) generation was higher than that of plantlets germinated from seeds.
Keywords:Vicia faba  rapid propagation  heat and ultraviolet stress  chromosomal aberration
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