烟碱和芸香苷对斜纹夜蛾药剂敏感性及相关酶活性的影响

以在人工饲料中添加植物次生物质的方法,研究了取食不同含量烟碱、芸香苷的斜纹夜蛾Spodoptera litura 3龄幼虫对银纹夜蛾核多角体病毒(AucaNPV)、氟虫腈及多杀菌素的敏感性,同时测定了各处理组幼虫体内羧酸酯酶和谷胱甘肽转移酶的活性。结果表明:烟碱能明显提高斜纹夜蛾对3种药剂的敏感性,且与剂量成正相关;低浓度的芸香苷可增加试虫对药剂的敏感性,但随其剂量的升高敏感性降低。两种次生物质均能不同程度地抑制斜纹夜蛾羧酸酯酶的活性,但可诱导谷胱甘肽转移酶的活性。

Effects of Nicotine and Rutin on the Susceptibility of Spodoptera litura to Insecticides and the Activities of Some Enzymes

The susceptibilities of the 3th instar larvae of Spodoptera litura fed on diets treated with nicotine and rutin to AucaNPV,fipronil and spinosad were studied.The activities of CarE and GSTs in different treated cutworms were also tested.The results showed that the nicotine enhanced significantly the susceptibility of Spodoptera litura to insecticides,and the susceptibility enhanced as the nicotine concentration in the diets increased.Low concentration rutin in the diets increased the susceptibility of Spodoptera litura to insecticide,but the susceptibility decreased as the concentration increased.The two alleochemicals restrained the activities of CarE in different degrees,however,they induced the activities of GSTs.