| 非洲猪瘟病毒pS273R蛋白水解酶单克隆抗体制备 |
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| 引用本文： | 张萌,刘英楠,谢振华,敖清莹,吕璐,于婉琪,金文杰,秦爱建,扈荣良,陈鸿军. 非洲猪瘟病毒pS273R蛋白水解酶单克隆抗体制备[J]. 畜牧兽医学报, 2021, 52(3): 839-844. DOI: 10.11843/j.issn.0366-6964.2021.03.028 |
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| 作者姓名： | 张萌 刘英楠 谢振华 敖清莹 吕璐 于婉琪 金文杰 秦爱建 扈荣良 陈鸿军 |
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| 作者单位： | 1. 中国农业科学院上海兽医研究所, 上海 200241;2. 军事科学院军事医学研究院军事兽医研究所, 长春 130122;3. 扬州大学兽医学院, 扬州 225009 |
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| 基金项目： | “十三五”国家重点研发专项资助项目(2017YFD0502300,2018YFC08400400);自然科学基金联合基金项目重点支持项目-区域创新发展联合基金项目(U19A2039)。 |
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| 摘 要： | 本研究旨在制备非洲猪瘟病毒(ASFV)S273R蛋白的特异性单克隆抗体.本研究以原核表达的非洲猪瘟病毒重组S273R蛋白免疫BALB/c小鼠,取其脾细胞与骨髓瘤细胞进行融合获得杂交瘤细胞.结果显示:基于纯化的S273R蛋白建立的间接ELISA方法对杂交瘤细胞进行筛选和亚克隆,获得了 4株可稳定分泌抗ASFV S273R...
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| 关 键 词： | 非洲猪瘟病毒 S273R蛋白 原核表达 单克隆抗体 |
| 收稿时间： | 2020-06-28 |
Preparation of Monoclonal Antibodies against African Swine Fever Virus pS273R Protease |
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| ZHANG Meng,LIU Yingnan,XIE Zhenhua,AO Qingying,Lü Lu,YU Wanqi,JIN Wenjie,QIN Aijian,HU Rongliang,CHEN Hongjun. Preparation of Monoclonal Antibodies against African Swine Fever Virus pS273R Protease[J]. Chinese Journal of Animal and Veterinary Sciences, 2021, 52(3): 839-844. DOI: 10.11843/j.issn.0366-6964.2021.03.028 |
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| Authors: | ZHANG Meng LIU Yingnan XIE Zhenhua AO Qingying Lü Lu YU Wanqi JIN Wenjie QIN Aijian HU Rongliang CHEN Hongjun |
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| Affiliation: | 1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;2. Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun 130122, China;3. Yangzhou University, Yangzhou 225009, China |
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| Abstract: | The objective of this study was to prepare monoclonal antibodies against the S273R protein of African swine fever virus(ASFV).BALB/c mice were immunized with purified recombinant S273R protein expressed by prokaryotic expression system and hybridoma cells were obtained by fusion of spleen cells and myeloma cells.An indirect ELISA based on the purified S273R protein was developed.Four hybridoma cell lines stably secreting MAbs against the S273R protein were harvested after screening and subcloning,and their sensitivity and specificity were detected by ELISA,Western blot,and immunofluorescence assay(IFA).Four MAbs heavy chains belong to IgG1,and light chains were κ.This research provided technical support for further study of biological functions of ASFV pS273R,as well as its gene-deletion based vaccine development. |
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| Keywords: | African swine fever virus S273R prokaryotic expression monoclonal antibody |
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