河南省小麦黑胚病优势病原菌链格孢菌(Alternaria spp.)遗传多样性分析

 采用RAPD技术对分离自河南省各地的43株小麦黑胚病优势病原菌(Alternaria spp.)菌株进行了遗传多样性分析。17个随机引物共扩增出151条清晰的DNA条带,所扩增出的DNA条带均为多态带,说明河南省小麦黑胚病菌存在着丰富的遗传多样性。利用NTSYS软件进行了病原菌的聚类分析,结果表明,河南省小麦黑胚病菌主要有2个种,即Alternaria alternata和A. tenuissima,种间的遗传相似系数的变化幅度为0.62~0.92。来自同一个地区的小麦黑胚病菌菌株基本上聚在了一起,表现出很近的亲缘关系,来自不同地区之间的菌株也可以交叉聚类。病原菌遗传多样性分析进一步验证了形态学的鉴定结果。

Analysis on genetic diversity of the dominant pathogens of wheat black point Alternaria spp. in Henan

The genetic diversity of 43 Alternaria spp. isolates from the wheat black point of six representative areas in Henan Province was analyzed by RAPD. 151 RAPD markers were obtained, which were amplified by 17 random primers, with 100% polymorphisms. It showed that rich genetic diversity of the pathogens of wheat black point was existed in Henan Province. The results of NTSYS cluster analysis showed that Alternaria alter-nata and A. tenuissima were the main pathogens of wheat black point, and the similarity coefficients were ranged from 0.62 to 0.92. Most of the isolates from the same area were clustered together and showed close genetic relationships. Some isolates from different areas were also clustered together with similarity. The results of genetic diversity analysis to the isolates had basically proved the morphological identification.