| 毕赤酵母表达MSL的发酵条件研究 |
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| 引用本文： | 李海涛,苗利光,刘艳环,王文昊,王松,安亚雄.毕赤酵母表达MSL的发酵条件研究[J].特产研究,2011,33(4):11-15. |
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| 作者姓名： | 李海涛 苗利光 刘艳环 王文昊 王松 安亚雄 |
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| 作者单位： | 中国农业科学院特产研究所,吉林吉林,132109 |
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| 基金项目： | 吉林省科技发展计划项目 |
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| 摘 要： | 本试验对含有pPIC9K-MSL转化子的毕赤酵母在BSM基础盐培养基中发酵培养条件进行了研究,并从BSM基础盐培养基组分、补料方式以及诱导时间对酵母生长发酵和表达高浓度的外源蛋白的影响进行了单因素优化试验,得到了毕赤酵母在BSM基础盐中发酵MSL的生产工艺。
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| 关 键 词： | 发酵 毕赤酵母 |
Studies on Fermentation Conditions for MSL Production by Pichia pastoris |
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| LI Hai-tao,MIAO Li-guang,LIU Yan-huan,WANG Wen-hao,WANG Song,AN Ya-xiong.Studies on Fermentation Conditions for MSL Production by Pichia pastoris[J].Special Wild Economic Animal and Plant Research,2011,33(4):11-15. |
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| Authors: | LI Hai-tao MIAO Li-guang LIU Yan-huan WANG Wen-hao WANG Song AN Ya-xiong |
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| Institution: | LI Hai-tao,MIAO Li-guang,LIU Yan-huan,WANGWen-hao,WANG Song,AN Ya-xiong (Institute of Special Wild Economic Animals and Plants,CAAS,Jilin 132109,China) |
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| Abstract: | In this article,we investigated the fermentation conditions for MSL(pPIC9K-MSL/GS115)production based on the base salt medium(BSM) by pichia pastoris.For the optimal fermentation conditions of Pichia pastoris growth and high level expression MSL,we made several rectifications in the components of medium,feed modes and induction time.We obtained the productive technology for MSL production by pichia pastoris in BSM. |
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| Keywords: | Fermentation Pichia pastoris |
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