| 红参中外源糖含量的测定 |
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| 引用本文： | 张浩,许世泉,张瑞,陈少麟,王英平.红参中外源糖含量的测定[J].特产研究,2011,33(4):56-59. |
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| 作者姓名： | 张浩 许世泉 张瑞 陈少麟 王英平 |
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| 作者单位： | 1. 中国农业科学院特产研究所,吉林吉林,132109
2. 抚松县人参研究所,吉林抚松,134500 |
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| 摘 要： | 通过对紫外吸收光谱、试剂用量、显色温度等因素进行考查,确定苯酚-硫酸法测定红参单寡糖含量的最佳试验条件。结果表明,5%苯酚1mL、硫酸7mL、60℃保温30min、检测波长490nm时,葡萄糖检测浓度在20~120μg/mL范围内与吸光度呈良好的线性关系（R2=0.999 3）,平均回收率100.3%,RSD=2.99%。该方法操作简便,精密度、重现性、稳定性良好,测得加糖红参中单寡糖含量是未加糖红参（对照）的2~4倍,可用于红参外源糖的检测。
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| 关 键 词： | 苯酚-硫酸 红参 多糖 |
Study on Detection of Exogenous Sugars of Red Ginseng |
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| ZHANG Hao,XU Shi-quan,ZHANG Rui,CHEN Shao-lin,WANG Ying-ping.Study on Detection of Exogenous Sugars of Red Ginseng[J].Special Wild Economic Animal and Plant Research,2011,33(4):56-59. |
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| Authors: | ZHANG Hao XU Shi-quan ZHANG Rui CHEN Shao-lin WANG Ying-ping |
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| Institution: | ZHANG Hao1,XU Shi-quan1,ZHANG Rui1,CHEN Shao-lin2,WANG Ying-ping1 (1.Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Jilin 132109,China,2.Fusong Institute of Ginseng,Fusong 134500,China) |
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| Abstract: | In order to determine the optimum testing conditions,the wave length,reagents and reaction temperature etc were investigated.Results showed that when the 1.0mL of 5% phenol aqueous solution and 7.0mL of concentrated sulfuric acid were used to complete the reaction at 60℃,glucose had a good liner relationship(R2=0.993) with absorbency ranged from20 to 120μg/mL,and with an average recovery rate of 100.3%,RSD= 2.99%.Total content of oligosaccharides in red ginseng (added sugar) was 2~4 times higher than CK.This method,with good precision,reproducibility and stability,was simple and convenient,and was suitable for detection of red ginseng foreign sugar. |
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| Keywords: | Phenol-sulfuric acid Red ginseng Polysaccharide |
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