| 苦马豆素人工抗原的合成及其免疫原性的研究 |
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| 引用本文： | 王帅,陈根元,吴书奇,张玲,马春晖,胡建军. 苦马豆素人工抗原的合成及其免疫原性的研究[J]. 塔里木大学学报, 2011, 23(3): 46-53. DOI: 10.3969/j.issn.1009-0568.2011.03.009 |
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| 作者姓名： | 王帅 陈根元 吴书奇 张玲 马春晖 胡建军 |
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| 作者单位： | 新疆生产建设兵团塔里木畜牧科技重点实验室/塔里木大学动物科学学院,新疆阿拉尔,843300 |
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| 基金项目： | 新疆生产建设兵团博士资金专项，国家自然科学基金项目，新疆生产建设兵团塔里木畜牧科技重点实验室项目 |
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| 摘 要： | 本实验用溴乙酸制备的活性酯与苦马豆素（SW）反应得到季铵盐，与牛血清白蛋白（BSA）偶联后，产物经透析、冻干后呈白色片状结晶。通过化学检验，制备的结晶为SW—BSA人工抗原，经SDS—PAGE电泳分析，分子量为29．0Kda，平均每个牛血清蛋白分子上结合47个季铵盐分子。将SW—BSA免疫小鼠后，采用间接ELISA实验检测血清抗体效价，结果表明合成的人工抗原具有较好的免疫原性，为动物小花棘豆中毒的防治奠定了基础。
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| 关 键 词： | 苦马豆素 人工抗原 合成 鉴定 |
The Study on Synthesis of Artificial Antigens of Swainsonine and Its Immunogenicity |
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| Wang Shuai,Chen Genyuan,Wu Shuqi,Zhang Ling,Ma Chunhui,Hu Jianjun. The Study on Synthesis of Artificial Antigens of Swainsonine and Its Immunogenicity[J]. Journal of tarim University, 2011, 23(3): 46-53. DOI: 10.3969/j.issn.1009-0568.2011.03.009 |
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| Authors: | Wang Shuai Chen Genyuan Wu Shuqi Zhang Ling Ma Chunhui Hu Jianjun |
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| Affiliation: | Wang Shuai Chen Genyuan Wu Shuqi Zhang Ling Ma Chunhui Hu Jianjun(Key laboratory of Tarim Animal Husbandry Science and Technology,XinJiang Production & Construction Corps / College of Animal Science,Alar,XinJiang 843300) |
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| Abstract: | In this experiment the quaternary salt was synthesized from the swainsonine and active ester which prepared from bromoacetic acid,Then it was coupled with the BSA.The product was dialysed and lyophilized to obtain crystal of white needle.SDS-PAGE analysis showed that the molecular weight of the product was 59.5 KDa.Each BSA molecule combined 47 molecules of quaternary salt after determination.Then the mice were immunized with SW-BSA,the serum titers were detected using indirect ELISA.The result showed that ... |
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| Keywords: | Swainsonine artificial antigens synthesis dentification |
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