草珊瑚试管苗生根培养研究 |
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引用本文: | 朱淑颖,张海洋,杨志红,张明永. 草珊瑚试管苗生根培养研究[J]. 安徽农业科学, 2010, 38(28): 15545-15547,15563. DOI: 10.3969/j.issn.0517-6611.2010.28.038 |
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作者姓名: | 朱淑颖 张海洋 杨志红 张明永 |
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作者单位: | 湖州师范学院生命科学学院,浙江湖州,313000;中国科学院华南植物园,广东广州,510650 |
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基金项目: | 湖州市科技计划项目,钱江人才计划项目 |
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摘 要: | [目的]建立有效的草珊瑚[Sarcandra glabra(Thunb.)Nakai]试管苗生根系统,以完善组培体系。[方法]以草珊瑚组培再生芽为供试材料,研究了不同生长素种类及浓度、不同无机盐和蔗糖浓度对其不定根形成的影响。[结果]NAA不利于草珊瑚的生根诱导,IBA可促进生根,提高生根速度和根系质量,浓度以0.20mg/L最佳;较低浓度无机盐更有利于草珊瑚生根培养,如1/2MS或1/4MS;蔗糖浓度在30g/L时诱导草珊瑚生根效果最好,过高或过低都会抑制其生根。[结论]草珊瑚试管苗生根培养的适宜培养基为1/2MS+IBA0.20mg/L+蔗糖30g/L或1/4MS+IBA0.20mg/L+蔗糖30g/L。
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关 键 词: | 草珊瑚 生根 组织培养 |
Study on Rooting Cultre of Sarcardra glabra (Thunb.)Nakai in vitro |
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Affiliation: | ZHU Shu-ying et al (College of Life Science, Institute of Cell Engineering, Huzhou Teachers' College, Huzhou, Zhejiang 313000) |
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Abstract: | [Objective]The research aimed to establish an efficient rooting protocol in vitro for perfecting the tissue culture system of Sarcandra glabra (Thunb.) Nakai.[Method]With the regenerated buds of S.glabra (Thunb.) Nakai as tested materials,the effects of auxin,mineral strength and sucrose concentration on adventitious root formation were studied.[Result]NAA went against the rooting induction of S.glabra (Thunb.) Nakai.IBA could promote in vitro rooting for improving the rooting speed and the root system quality.The optimum concentration was 0.20 mg/L.Lower mineral strength such as 1/2MS or 1/4MS was much better for rooting of S.glabra (Thunb.) Nakai.The best induction results were obtained at the concentration of 30 g/L.Too high or too low sucrose concentration had some restrainable effect on root formation.[Conclusion]The optimal rooting media of S.glabra (Thunb.) Nakai was 1/2MS+0.20 mg/L IBA+30 g/L sucrose and 1/4 MS+0.20 mg/L IBA + 30 g/L sucrose. |
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Keywords: | Sarcardra glabra (Thunb.) Nakai Rooting Tissue culture |
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