湖南省部分地区棉田牛筋草(Eleusine indica)对高效氟吡甲禾灵的抗药性

为制定棉田恶性杂草牛筋草的防除策略和延缓抗性种群发展,采用整株测定法测定了湖南省部分地区棉田杂草牛筋草(Eleusine indica)对高效氟吡甲禾灵的抗药性水平,并通过测定谷胱甘肽-S-转移酶(GST)活力研究了牛筋草对高效氟吡甲禾灵的代谢抗性机制。整株法测定结果显示,相对于敏感的鼎城区种群,不同地区牛筋草种群对高效氟吡甲禾灵均产生了不同程度的抗药性,抗药性指数在2.4~18.4。其中汉寿牛筋草种群的抗性水平最高,其次是大通湖、湘阴种群。汉寿抗性牛筋草种群GST活力明显高于鼎城区敏感种群,表明GST对高效氟吡甲禾灵代谢能力的差异是牛筋草对高效氟吡甲禾灵产生抗药性的一个重要原因。

Resistance of Eleusine indica to Haloxyfop-R-methyl in Cotton Fields in Part of Hunan Province

In order to evaluate the resistance of Eleusine indica populations to haloxyfop-R-methyl, whole-plant bioassay was measured, and the differences on GST of the resistant and susceptible populations to haloxyfop-R-methyl were preliminarily determined. The results of whole-plant bioassay showed that populations of Eleusine indica from the collecting sites of Hunan Province developed different levels of resistance to haloxyfop, and the highest resistant index(RI) was 18.4, which was found in populations from Hanshou. The next were that of Datonghu and Xiangyin population. The GST activity of resistant population decreased immediately and then increased rapidly at 5 days after treatment, with the peak value 9 days after, while that of susceptible population declined at first and then increased, then came down to the control value 9 days later. There were great difference between GST activities of Hanshou (resistant) and Dingcheng (susceptible) population, which indicated difference of GST metabolizing capacity is an important factor for Eleusine indica's resistance to haloxyfop-R-methyl.