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| 78份杧果种质亲缘关系的ISSR分析 | |
| 引用本文： | 应东山,罗海燕,黄建峰,彭素娜,陈业渊.78份杧果种质亲缘关系的ISSR分析[J].热带农业科学,2012,32(3):14-18. |
| 作者姓名： | 应东山 罗海燕 黄建峰 彭素娜 陈业渊 |
| 作者单位： | 1. 海南大学园艺园林学院,海南海口520228／中国热带农业科学院热带作物品种资源研究所／农业部热带作物品种资源利用重点开放实验室海,南儋州571737 2. 中国热带农业科学院热带作物品种资源研究所／农业部热带作物品种资源利用重点开放实验室海,南儋州,571737 3. 广西南亚热带农业科学研究所,广西龙州,532415 |
| 基金项目： | 中国热带农业科学研究院热带作物品种研究所导师基金(No.DSJJ2010-04);海南省自然科学基金“我国地方芒果种质资源的指纹图谱构建”(No.3100879) |
| 摘 要： | 采用ISSR分子标记技术,分析78份杧果种质的遗传多样性。从100条引物中筛选出12条优选引物,共扩增出148条DNA条带,其中多态性带142条,多态性百分率为95.95%。聚类分析结果将78份杧果种质分为5大类。在5大类资源中,大部分种质(87.34%)都聚在第Ⅰ类,表明大部分杧果种质之间亲缘关系较近,供试种质之间能互相区分开来并表现出丰富的遗传多样性。 |
| 关 键 词： | 杧果 种质资源 遗传多样性 聚类分析 |
ISSR Analysis of 78 Mango Germplasm Genetic Relationships |
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| YING Dongshan,LUO Haiyan,HUANG Jianfeng,PENG Suna,CHEN Yeyuan.ISSR Analysis of 78 Mango Germplasm Genetic Relationships[J].Chinese Journal of Tropical Agriculture,2012,32(3):14-18. | |
| Authors: | YING Dongshan LUO Haiyan HUANG Jianfeng PENG Suna CHEN Yeyuan |
| Institution: | 1 College of Horticulture and Landscape Architecture,Hainan University,Haikou,Hainan 520228; 2 Tropical Crops Genetic Resources Institute/Ministry of Agriculture Key Laboratory of Tropical Crops Germplasm Resources Utilization,CATAS,Danzhou,Hainan 571737; 3 South Subtropical Agricultural Scientific Research Institute,Longzhou,Guangxi 532415) |
| Abstract: | ISSR marker was used to analyze genetic diversities among 78 mango germplasm.12 primers selected from 100 ISSR-primers were used for amplification and a total of 148 DNA bands were obtained,including 142 polymorphic bands.The percent of polymorphic is 95.95%.The result showed that the cluster analysis based on ISSR data classified the mango germplasm into 5 major groups.In these groups,most of the samples(87.34%) were gathered in groupⅠ,indicating most of the mango germplasm with close genetic relationship.In this study,all cultivars tested were distinguished from each other and showed ample genetic diversity,indicating that ISSR-PCR was an effective method for cultivar identification of mangoes. |
| Keywords: | mango germplasm resources genetic diversity cluster analysis |
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