海马齿SpSCL1基因启动子克隆及序列分析

GRAS蛋白是植物特有的一类蛋白家族,在植物的生长发育方面具有重要作用.根据前期分离的海马齿SpSCL1基因的5'端序列,设计特异引物进行Genome Walking,从海马齿基因组DNA中克隆了SpSCL1基因上游的1 254 bp片段.序列分析表明,该序列包含681 bp的启动子调控序列及573 bp的基因编码序列,启动子调控序中除含有典型的真核生物核心启动子区域(-60～-10bp)外,还含有多个TATA-box、CAAT-box等启动子元件以及多种与脱水、耐盐和光响应相关的顺式作用元件.结果预示海马齿SpSCL1可能在抗旱、耐盐以及光敏色素信号转导途径中发挥重要作用.

Cloning and sequence analysis of SpSCL1 promoter from Sesuvium portulacastrum

The GRAS proteins are a recently identified family of plant-specific proteins, which play a crucial role in diverse plant growth and development. Specific primers was designed according to the 5''region of SpSCL1 gene from Sesuvium portulacastrum, the 1 254 bp 5''regulatory sequence of SpSCL1 was cloned from shoot genomic DNA of Sesuvium portulacastrum by Genome Walking method. Sequence analysis showed the sequence contains 681 bp of the promoter regulation sequence and the gene coding region sequence of 573 bp, the promoter regulation sequence consisted of a typical core promoter region of eukaryotic (-60~-10 bp), several promoter element such as TATA-box and CAAT-box, as well as many cis-regulatory elements related to salt, dehydration and stress responses, there are also some cis-regulatory elements related to light responses. It suggested that SpSCL1 gene might play an important role in the salt tolerance, drought tolerance, phytochrome signal transduction pathways.