抱子甘蓝组织培养及人工种子研究

以抱子甘蓝下胚轴为外植体诱导再生植株,并制作人工种子。结果表明:苗龄为8d的无菌苗,其下胚轴诱导不定芽频率最高,平均诱导不定芽数达11.3个。6-BA、ZT对下胚轴诱导不定芽均具有明显的促进作用,6-BA2mg·L-1作用最显著,繁殖系数达11.2。不定芽在不含激素的1/2MS培养基上诱导生根,驯化移栽后成活。将不定芽用海藻酸钠包埋成人工种子,生根率为24%,移栽成活并开花。

Studies on Tissue Culture and Artificial Seeds of Brassica oleracea L. var. gemmifera Zenk.

The paper described the plantlet regeneration of Brassica oleracea from its hypocotyl and production of artificial seed with young shoots. The results showed that the hypocotyl segment from 8-day-old seedlings was the best for inducing adventitious buds and the averaged propagate coefficient of buds was 11.3. 6-BA and ZT significantly promoted to (inducing) adventitious buds, and the propagate coefficient was 11.2 under the 2 mg·L~(-1) of 6-BA. The adventitious buds were encapsulated with a sodium alginate ...