| 嗜水气单胞菌菌毛的提纯及特性分析 |
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| 引用本文： | 严亚贤,孙建和,陈怀青,陆承平. 嗜水气单胞菌菌毛的提纯及特性分析[J]. 南京农业大学学报, 1995, 0(3) |
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| 作者姓名： | 严亚贤 孙建和 陈怀青 陆承平 |
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| 作者单位： | 南京农业大学动物医学院，上海农学院 |
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| 摘 要： | 用电镜观察嗜水气单胞菌Ｊ－１株，见有两种不同的菌毛，一种为短而硬直的Ｒ菌毛，另一种为长而易弯曲的Ｗ菌毛。Ｒ菌毛数量多，周身分布；Ｗ菌毛数量少，一个菌体仅４～６根。搅拌菌体悬液可使菌毛脱落，低速离心的上清液经ＰＥＧ沉淀、硫酸铵盐析和Ｓｅｐｈａｒｏｓｅ４Ｂ层析后，获得纯化的Ｒ菌毛。ＳＤＳ－ＰＡＧＥ显示，在有２－巯基乙醇存在时，Ｒ菌毛的亚单位分子量为１７０００。氨基酸组分分析表明，Ｒ菌毛含有４１％的疏水性氨基酸。用纯化的Ｒ菌毛免疫家兔获抗血清，建立菌体凝集试验和Ｄｏｔ－ＥＬＩＳＡ法，检测５０个待检样本的Ｒ菌毛，两法检出率均超过６０％，符合率为８８％。
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| 关 键 词： | 嗜水气单胞菌，菌毛，形态特征，生化性状，免疫检测 |
PURIFICATION AND CHARACTERIZATION OF PILI FROM AEROMONAS HYDROPHILA |
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| Yan Yaxian, Sun Jianhe, Chen Huaiqing and Lu Chengping. PURIFICATION AND CHARACTERIZATION OF PILI FROM AEROMONAS HYDROPHILA[J]. Journal of Nanjing Agricultural University, 1995, 0(3) |
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| Authors: | Yan Yaxian Sun Jianhe Chen Huaiqing Lu Chengping |
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| Abstract: | Aeromonas hydrophila J-1 has 2 morphologically distinctive kinds of pill. One appears rigid and straight (R-pili). The other looks flexible and wavy (W-pill). R pill were purified by ammonium sulfate precipitation and Sepharase 4B column chromatography. The pill consists of a subunit protein with a molecular weightof 17 000 as estimated by SDS-PAGE and contains 41% hydrophobic amino acids. Cellagglutination test and Dot-ELISA with antiserum aganist R pill for the detection of aeromonas pill are develope, and the positive rates of both methods are extremely similar. |
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| Keywords: | Aeromonas hydrophila pili morphological characteristics biochemical traits immunoassay |
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