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转Bt基因水稻克螟稻杂交转育后代农艺性状的研究
引用本文:崔海瑞,王忠华,舒庆尧,吴殿星,夏英武,高明尉.转Bt基因水稻克螟稻杂交转育后代农艺性状的研究[J].中国水稻科学,2001,15(2):101-106.
作者姓名:崔海瑞  王忠华  舒庆尧  吴殿星  夏英武  高明尉
作者单位:浙江大学原子核农业科学研究所,浙江杭州 310029
基金项目:浙江省科委“九五”重大项目;美国洛氏基金资助项目(RF97001#556)。
摘    要: 对转Bt基因水稻与常规品种的杂交后代进行了GUS活性的组织化学分析和农艺性状考察。结果表明,报告基因gus与抗虫基因 cry1Ab是连锁遗传和协同表达的,利用GUS组织化学染色法可快速筛选抗虫杂种植株;无论是籼粳交,还是粳粳交后代,抗虫和感虫的两类植株间在株高、穗长、单株分蘖数、播始历期和千粒重等主要农艺性状上无显著差异,这无疑为利用Bt水稻培育优良的抗虫品种提供了科学依据。

关 键 词:水稻  农艺性状  杂交育种
文章编号:1001-7216(2001)02-0101-06
收稿时间:1900-01-01;

Agronomic Traits of Hybrid Progenies Between Bt Transgenic Rice and Conventional Rice Varieties
CUI Hai rui,WANG Zhong hua,SHU Qing yao ,WU Dian xing,XIA Ying wu,GAO Ming wei.Agronomic Traits of Hybrid Progenies Between Bt Transgenic Rice and Conventional Rice Varieties[J].Chinese Journal of Rice Science,2001,15(2):101-106.
Authors:CUI Hai rui  WANG Zhong hua  SHU Qing yao  WU Dian xing  XIA Ying wu  GAO Ming wei
Institution:CUI Hai rui,WANG Zhong hua,SHU Qing yao *,WU Dian xing,XIA Ying wu,GAO Ming wei
Abstract:GUS activity was analyzed by histochemical staining and agronomictraits were observed in hybrid progenies between transgenic Bt rice and conventional rice varieties. Results showed that reporter gene gus was co-inherited and co-expressed with the insect resistant gene cry1Ab and the GUS histochemical assay can be used to rapidly screen insect resistant plants. And no significant differences in main agronomic traits, such as plant height, panicle length, the number of tillers per plant, days to heading and 1000-grain weight, were found between the two classes of the insect resistant plant and the susceptible plant in the progenies of either indica/japonica crosses or japonica/japonica crosses, providing scientific basis for utilization of transgenic Bt rice in cross breeding for elite varieties with insect resistance.
Keywords:rice  agronomic trait  cross breeding  gene transfer
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