农杆菌介导玉米转基因的影响因素研究

研究了农杆菌介导玉米转基因过程中涉及的外植体选择、高渗和农杆菌共培养、选择培养基筛选转化体等因素对愈伤组织生长及转化效率的影响。结果表明,同一基因型玉米自交系中有少数幼穗幼胚诱导出的愈伤组织生长速度快,转化效率高;高渗和农杆菌共培养以及除草剂筛选抑制愈伤组织的生长,致使部分转基因愈伤不能分化成苗,并提出其解决途径。

Influencing factors on maize transgene process mediated by Agrobacterium.

The effects of explant selection,high osmotic treatment,co-culture with Agrobacterium,screening of transformed plants by selecting medium on the callus growth and transformation efficiency were studied in the process of maize transformation mediated by Agrobacterium.The results show that the growth rate and transformation efficiency of immature embryos derived from a vigorous ear are higher than those from other ears.High osmotic treatment and co-culture with Agrobacterium and herbicide screening impede the callus growth.The solutions are put forward.