| 用RAPD标记检测与杨树生长和物候期有关的QTLs |
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| 引用本文： | 本文为1995年林业部指南项目“杉木、杨树遗传图谱构建及数量性状位点(QTL)定位研究”和1997年国家“九五”攀登计划项目“人工林木材性质及其生物形成与功能性改良机理的研究”的一部分。. 用RAPD标记检测与杨树生长和物候期有关的QTLs[J]. 林业科学研究, 1999, 12(2): 2-3 |
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| 作者姓名： | 本文为1995年林业部指南项目“杉木、杨树遗传图谱构建及数量性状位点(QTL)定位研究”和1997年国家“九五”攀登计划项目“人工林木材性质及其生物形成与功能性改良机理的研究”的一部分。 |
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| 作者单位： | 中国林业科学研究院林业研究所，加拿大多伦多大学林学系 |
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| 基金项目： | 1995年林业部指南项目,1997年国家“九五”攀登计划项目 |
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| 摘 要： | 取美洲黑杨（母本）和青杨（父本）杂交获得的Ｆ２群体样本８０株，应用ＲＡＰＤ标记检测与Ｆ２群体３个数量性状（苗高、地径和封顶期）有关的ＱＴＬｓ。在Ｆ２群体中，一年生苗高、地径和封顶期性状表现显著分离，基本符合正态分布。单因子方差分析检测出与苗高、地径和封顶期性状相关的７个、６个和３个标记座位，并计算了各标记对相关性状变异的贡献率。与数量性状相关的标记有不同的亲本来源。ｔ检验结果表明，各标记显带型和不显带型组性状均值的差异显著或极显著（两个标记除外）；与各性状相关标记的显带型或不显带型的频率，在各性状上的分布有异同。双因子方差分析结果表明，在与性状相关联的标记中，有两组ＱＴＬｓ互作组合与苗高性状相关联。
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| 关 键 词： | 美洲黑杨；青杨；F_2群体；RAPD标记；数量性状基因座位（QTL） |
Detectionof QTLs for Growth and Phenology Traits of Poplar Using RAPD Markers |
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| Li Jinhua)Su Xiaohua)Zhang Qiwen)Louis Zsuffa)T. Detectionof QTLs for Growth and Phenology Traits of Poplar Using RAPD Markers[J]. Forest Research, 1999, 12(2): 2-3 |
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| Authors: | Li Jinhua)Su Xiaohua)Zhang Qiwen)Louis Zsuffa)T |
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| Affiliation: | Li Jinhua1)Su Xiaohua1)Zhang Qiwen1)Louis Zsuffa2)T |
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| Abstract: | The objective of the study is to investigate QTLs associations with three quantitative traits (height,diameter and top closure) within 80 F2 seedlings of a cross between a Populus deltoides female and a P.cathayana male by using RAPD makers. Singlefactor analysis of variance was used to determine the significance of difference among genotypic classes. Seven, six and three markers were respectively found to be associated with height,diameter and top closure,which jointly explained 45.94%,41.17% and 19.13% of the total phenotypic variance.Twoway analysis of variance was used to detect the significance of interaction between putative QTLs. Two interactions of three markers were found to be associated with height, and each explained 4.57% and 5.05% of the phenotypic variance respectively.One of the three markers was associated with height,diameter and top closure,one was associated with diameter and the other with top closure. |
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| Keywords: | Populus deltoides P.cathayana F2 population RAPD Quantative Trait Loci (QTL) |
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