| 酶解条件对黄瓜原生质体分离效果的影响 |
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| 引用本文： | 岳备,黄玉兰,林晓婷,耿士庄,赵国峰,梁晓庆,刘延明.酶解条件对黄瓜原生质体分离效果的影响[J].安徽农业科学,2014(19):6145-6147. |
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| 作者姓名： | 岳备 黄玉兰 林晓婷 耿士庄 赵国峰 梁晓庆 刘延明 |
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| 作者单位： | 黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319;黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319;黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319;黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319;黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319;黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319;黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319 |
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| 基金项目： | 黑龙江省青年科学基金(QC2012C103);校级大学生创新创业训练计划项目(2013100223003) |
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| 摘 要： | 目的]研究黄瓜原生质体分离的最佳酶解条件.方法]以黄瓜子叶和悬浮细胞为材料,研究不同酶解条件对其原生质体的分离效果的影响.结果]黄瓜子叶获得高质量的原生质体的最佳条件为：酶液组合2％纤维素酶＋1.0％果胶酶,酶解时间8h,酶液浓度0.7 mol/L,酶液pH 5.5.结论]该试验研究了不同酶解条件对黄瓜原生质体的分离效果的影响,为黄瓜的进一步开发利用提供依据.
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| 关 键 词： | 黄瓜(Cucumis sativus L.) 原生质体 酶解条件 离心 |
The Effect of Decomposing Conditions on Protoplast Isolation of Cumber |
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| Institution: | YUE Bei, HUANG Yu-lan et ai (Faculty of Biological Life, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319) |
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| Abstract: | Objective] To study the optimal enzymolysis conditions on protoplast isolation of cumber.Method] Using cotyledons and suspension of cumber as test materials,effects of various enzymolysis conditions on protoplast isolation were studied.Result] The optimum conditions for high yield of viable protoplast were as follows：enzyme combination containing 2% cellulase ＋ 1.0% pectinase,mannitol concentration at 0.7 mol/L,enzymolysis time of 8 h and pH =5.5.Conclusion] Effects of different enzymolysis conditions on protoplast isolation of cumber were studied,which will provide basis for further development and utilization. |
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| Keywords: | Cucumis sativus L Protoplast Enzymolysis conditions Centrifugation |
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