| 新城疫病毒融合蛋白基因片段的构建与鉴定 |
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| 引用本文： | 贺东生,刘福安,宋长绪,吴红专,王星. 新城疫病毒融合蛋白基因片段的构建与鉴定[J]. 广西农业生物科学, 1998, 0(3) |
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| 作者姓名： | 贺东生 刘福安 宋长绪 吴红专 王星 |
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| 作者单位： | 华南农业大学动物医学系!广州510642 |
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| 基金项目： | 国家教育部博士点基金课题资助项目 !(编号 940 5 0 7) |
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| 摘 要： | 本文重组和构建了新城疫病毒 ( NDV)融合蛋白基因 ,并对该基因进行了鉴定 :将新城疫病毒 F基因片段经 RT— PCR扩增 ,插入经 Eco R I/ Sal 酶切的克隆载体 p UC18及表达载体 p GEMEX,转化大肠杆菌 JM10 9株。用氨苄青霉素平板法初步筛选克隆 ,再用双酶切法、核酸探针、 PCR及核苷酸序列分析法鉴定 ,表明插入成功并且阅读框架正确
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| 关 键 词： | 新城疫病毒 融合蛋白基因片段 构建 鉴定 |
Construction and Characterization of Fusion Protein Gene Fraction of Newcastle Disease Virus |
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| He Dongsheng L iu Fuan Song Changxu Wu Hongzhuan Wang Xing. Construction and Characterization of Fusion Protein Gene Fraction of Newcastle Disease Virus[J]. Journal of Guangxi Agricultural and Biological Science, 1998, 0(3) |
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| Authors: | He Dongsheng L iu Fuan Song Changxu Wu Hongzhuan Wang Xing |
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| Abstract: | A pair of polymerase chain reaction(PCR) primers coding the Newcastle dis- ease virus (NDV) Fx gene were used to amplify a 45 3bp NDV Fx by RT PCR. The Fx DNA was transformed into Escherichia coli JM1 0 9after being inserted into p UC1 8.The re- combinant p NDV Fx was constructed and characterized successfully by the restrictive en- donuclease (RE) digesting,PCR,hybridization and nucleictide sequencing. |
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| Keywords: | Newcastle disease virus fusion protein gene fraction cloning characteriza- tion |
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