Recurrent phenotypic selection for forage yield in rye |
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Authors: | P. L. Bruckner P. L. Raymer G. W. Burton |
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Affiliation: | (1) Lehrstuhl für Allgemeine Genetik, Biologisches Institut der Universität Tübingen, Auf der Morgenstelle 28, D-7400 Tübingen, Germany;(2) Present address: Institut für Genetik und Mikrobiologie, Lehrstuhl für Genetik, Universität München, Maria Ward-Str. 1a, 8000 München, Germany |
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Abstract: | Summary Cultivars of Cucurbita pepo and other Cucurbita species were characterized by RFLP analysis using different fragments of the ribosomal intergenic spacer (IGS) of Cucurbita pepo as hybridization probes. Several cultivars could be distinguished by a specific rDNA restriction pattern, whereas some cultivars showed an identical RFLP pattern suggesting a closer relationship. Other species of the genus Cucurbita exhibited strong cross-reaction with the C. pepo spacer probes, in contrast to DNA of Cucumis species which did not cross-hybridize.Abbreviations IGS intergenic spacer - ITS internal transcribed spacer - kbp kilo base pairs - rDNA ribosomal DNA - RFLP restriction fragment length polymorphism - rRNA ribosomal RNA |
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Keywords: | Cucurbitaceae Cucurbita spec. intergenic spacer plant breeding restriction fragment length polymorphism (RFLP) ribosomal DNA (rDNA) |
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