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| 绢丝昆虫樟蚕线粒体全基因组克隆及序列分析 | |
| 引用本文： | 洪桂云,姜绍通,于淼,杨英,刘彦群,魏兆军.绢丝昆虫樟蚕线粒体全基因组克隆及序列分析[J].核农学报,2010,24(3):495-501. |
| 作者姓名： | 洪桂云 姜绍通 于淼 杨英 刘彦群 魏兆军 |
| 作者单位： | 1. 合肥工业大学生物与食品工程学院,安徽合肥,230009;安徽建筑工业学院环境工程系,安徽合肥,230601 2. 合肥工业大学生物与食品工程学院,安徽合肥,230009 3. 沈阳农业大学生物科学与生物技术学院,辽宁沈阳,110161 |
| 基金项目： | 教育部新世纪优秀人才基金项目，安徽省优秀人才基金项目，国家自然科学基金项目 |
| 摘 要： | 采用Long-PCR方法,克隆并测定了樟蚕(Eriogyna pyretorum)线粒体基因组全序列。樟蚕线粒体基因组全序列为15,327bp,基因组碱基组成为39.17%A,11.55%C,7.63%G,41.65%T,共有13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个AT富含区(A+T-rich区)。樟蚕线粒体基因组结构与鳞翅目其他昆虫相同。除cox1和cox2基因外,其他蛋白编码基因含有典型的ATN起始密码子;13个蛋白质编码基因中,有11个使用TAA作为终止密码子。预测了22个tRNA基因的二级结构,发现除trnS1(AGN)缺少DHU臂外,其他的tRNA具有典型的三叶草结构。 |
| 关 键 词： | 绢丝昆虫 樟蚕 鳞翅目 蚕蛾总科 线粒体基因组 |
CLONING AND SEQUENCING ANALYSIS OF THE COMPLETE MITOCHONDRIAL GENOME OF Eriogyna pyretorum |
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| HONG Gui-yun,JIANG Shao-tong,YU Miao,YANG Ying,LIU Yan-qun,WEI Zhao-jun.CLONING AND SEQUENCING ANALYSIS OF THE COMPLETE MITOCHONDRIAL GENOME OF Eriogyna pyretorum[J].Acta Agriculturae Nucleatae Sinica,2010,24(3):495-501. | |
| Authors: | HONG Gui-yun JIANG Shao-tong YU Miao YANG Ying LIU Yan-qun WEI Zhao-jun |
| Institution: | 1.School of Biotechnology and Food Engineering, Hefei University of Technology,Hefei, Anhui 230009; 2.Department of Environmental engineering, Anhui University of Architecture, Hefei, Anhui 230601; 3.College of Bioscience and Biotechnology, Shenyang Agricultural University, Liaoning, Shenyang 110161 |
| Abstract: | The complete mitochondrial genome of E. pyretorum was cloned using long-PCR amplification. The mitochondrial genome of E. pyretorum was 15, 327bp. The base compositions included 39.17% A, 11.55% C, 7.63% G, 41.65% T, and consisted of 13 protein coding gene (PCGs), 2 rRNAs and 22 tRNA genes and one A+T-rich region. All PCGs of the E. pyretorum mitochondrial genome started with a typical ATN codon, except for cox1 and cox2. The usual TAA termination codon was found for eleven PCGs. The arrangement of PCGs was the same as that found in other sequenced lepidopteran insect. However, cox1 and cox2 genes had an incomplete termination codon (T). With respect to the secondary structures of 22 tRNA genes, the absence of DHU arm of trnS1 (AGN) was observed in E. pyretorum. |
| Keywords: | silk-producing insects Eriogyna pyretorum Lepidoptera Saturniidae mitochondrial genome |
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