马铃薯杂种优良株系遗传差异的AFLP分析

为明确从‘1867’ב陇薯7号’、‘MB09’ב陇薯7号’和‘MB09’ב陇薯6号’3个马铃薯杂交组合中选育出的17个杂种优良株系的遗传差异程度,用4个亲本材料作对照,利用AFLP分子标记技术对其进行了遗传差异分析。用筛选出的10对AFLP适宜引物进行PCR扩增共得到321个位点,多态性位点277个,多态性位点百分率87.29%。试验建立了各杂种优良株系的AFLP指纹图。21份马铃薯材料间的多态信息含量(PIC)平均为0.5617,Nei’s遗传多样性指数为0.3623,Shannon指数为0.5407。各材料间的平均遗传距离(GD)为0.5281,以GD值0.51为基准,21份材料分为4类:‘1867’、A-10、A-12、A-21和A-29为一类;‘陇薯6号’、‘陇薯7号’、A-14、A-23、A-26、B-13和B-14为一类;‘MB09’、B-1、B-2、B-7、B-15、B-20和C-22为一类;C-2和C-21为一类。该研究可为马铃薯杂交亲本的选择利用和杂种优良新品系的选育提供依据。

AFLP analysis of genetic differences among elitehybrid potato lines

To identify heredity differences among 17 elite hybrid lines selected from three cross combinations of‘1867’בLongshu.7’,‘MB09 ’בLongshu.7’and ‘MB09 ’בLongshu.6 ’potatoes,we used four parent lines as controls and analyzed genetic differences using AFLP molecular markers.A total of 321 loci were am-plified using 10 AFLP primer pairs,of which 277 were polymorphic,accounting for 87.29% of all loci.The experiment established an AFLP fingerprint map of elite hybrid lines.The average polymorphism information content (PIC)from 21 samples was 0.5617;Nei’s gene diversity index was 0.3623 and the Shannon informa-tion index was 0.5407.The average genetic distance (GD)of the tested lines was 0.5281 and were classified in-to four categories based on a GD value of 0.51:‘1867’,A-10,A-12,A-21 and A-29;‘Longshu.6’,‘Long-shu.7’,A-14,A-23,A-26,B-13 and B-14;‘MB09’,B-1,B-2,B-7,B-15,B-20 and C-22,and C-2 and C-21. This study provided a basis for selection and utilization of potato hybrid parents and breeding of new hybrid va-rieties.