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| 宗地花猪H-FABP基因mRNA的表达与肉质性状的相关研究 | |
| 引用本文： | 李平,燕志宏,陈伟,张芸,田松军. 宗地花猪H-FABP基因mRNA的表达与肉质性状的相关研究[J]. 中国畜牧兽医, 2015, 42(3): 644-649. DOI: 10.11843/j.issn.1671-7236.2015.03.021 |
| 作者姓名： | 李平 燕志宏 陈伟 张芸 田松军 |
| 作者单位： | 1. 贵州大学动物科学学院, 贵阳 550025; 2. 高原山地动物遗传育种与繁殖省部共建教育部重点实验室, 贵阳 550025; 3. 贵州省畜禽遗传资源管理站, 贵阳 550001; 4. 紫云县畜牧兽医局, 紫云 561000 |
| 基金项目： | 贵州省科技厅农业攻关项目(黔科合NZ字[2012]3013号);紫云宗地花猪生态养殖技术集成与产业化示范[黔科合县市科拨计划(2012)7032] |
| 摘 要： | 为研究不同饲养条件对地方猪种宗地花猪心脏型脂肪酸结合蛋白(heart fatty acid binding protein,H-FABP)基因表达及肌内脂肪(intramuscular fat,IMF)含量的影响,试验利用实时荧光定量PCR技术,分别测定放养型和圈养型宗地花猪背最长肌中H-FABP基因的表达水平,并测定背最长肌的IMF、大理石纹、嫩度.结果发现,圈养型宗地花猪的IMF、大理石纹、嫩度均高于放养型宗地花猪(P >0.05),pH、肉色极显著高于放养型宗地花猪(P <0.01);放养型宗地花猪背最长肌中H-FABP mRNA 水平显著高于圈养型宗地花猪(P <0.05);放养型、圈养型宗地花猪H-FABP基因表达与IMF均无显著相关性(P >0.05).以上结果表明,不同饲养条件影响H-FABP基因表达并在一定程度上影响IMF,但影响不显著. |
| 关 键 词： | 宗地花猪 H-FABP基因 肌内脂肪 |
| 收稿时间： | 2014-09-09 |
Association Analysis on H-FABP Gene Expression Level and Meat Quality Traits of Zongdi Spotted Pigs |
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| LI Ping;YAN Zhi-hong;CHEN Wei;ZHANG Yun;TIAN Song-jun. Association Analysis on H-FABP Gene Expression Level and Meat Quality Traits of Zongdi Spotted Pigs[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(3): 644-649. DOI: 10.11843/j.issn.1671-7236.2015.03.021 | |
| Authors: | LI Ping YAN Zhi-hong CHEN Wei ZHANG Yun TIAN Song-jun |
| Affiliation: | 1. College of Animal Science, Guizhou University, Guiyang 550025, China; 2. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; 3. The Management Station of Livestock and Poultry Genetic Resources in Guizhou Province, Guiyang 550001, China; 4. The Animal Husbandry and Veterinary in Ziyun County, Ziyun 561000, China |
| Abstract: | Different feeding conditions of Zongdi spotted pigs,H-FABP gene expression level was detected in back fat by Real-time PCR and IMF content in longissimus muscle of back was determined.The results showed that IMF,marble and tenderness of captivity type Zongdi spotted pigs were higher than grazing type (P >0.05),pH and meat color of captivity type Zongdi spotted pigs were very significantly higher than grazing type (P <0.01); H-FABP mRNA expression level of grazing type longissimus muscle of back was significantly higher than captivity type Zongdi spotted pigs (P <0.05);There was no significant correlation between H-FABP mRNA expression level and IMF content partly in grazing type and captivity type (P >0.05).Different feeding conditions could influence H-FABP mRNA expression and IMF content partly,yet influence was not significantly. |
| Keywords: | Zongdi spotted pigs H-FABP gene IMF |
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