活化卵白蛋白对断奶仔猪结肠内SCFA的影响

为了研究活化卵白蛋白对断奶仔猪结肠内短链脂肪酸(short chain fatty acid，SCFA)的影响，选取54头20日龄健康仔猪，随机分为3个处理组。23日龄开始，Ⅰ组饲喂基础日粮+活化卵白蛋白10 mL·d-1·头-1，Ⅱ组饲喂基础日粮+啤酒酵母10 mL·d-1·头-1，Ⅲ组连续饲喂基础日粮10 d，28日龄断奶。仔猪在23，28，35，42日龄时，从每个重复中随机选1头屠宰，迅速结扎结肠两端，无菌收集结肠食糜，利用气相色谱法测定结肠内SCFA含量。试验结果表明: (1)仔猪28日龄时，与Ⅲ组相比，Ⅰ组仔猪结肠内乙酸(P＜001)、丙酸(P＜005)、丁酸(P＜005)和总SCFA(P＜001)含量升高，而异丁酸(P＜001)含量降低；与Ⅱ组相比，Ⅰ组仔猪结肠内乙酸(P＜001)、异丁酸(P＜001)和总SCFA(P＜001)含量升高；(2)仔猪42日龄时，与Ⅱ组、Ⅲ组相比，Ⅰ组仔猪结肠内乙酸、丙酸、丁酸、异戊酸和总SCFA含量显著升高(P＜005)；(3)随着仔猪日龄的增加，各组仔猪结肠内总SCFA含量均增加，但Ⅰ组增加最明显。由此看来，活化卵白蛋白可以提高断奶仔猪结肠内SCFA含量。

Effects of active egg white protein on colonic short chain fatty acid of weaned piglets

To investigate the effects of active egg white protein on colonic short chain fatty acid(SCFA) of weaned piglets， fifty four 20 day old piglets were randomly divided into three groups， with three replications per group and six piglets each replication. Piglets in group Ⅲ were fed basal diet， the group Ⅰ and group Ⅱ respectively were fed basal diet supplemented with active egg white protein 10 mL·d-1 each piglet and Saccharomyces cerevisiae 10 mL·d-1 each piglet. From the beginning of 23 day old， the piglets in group Ⅰ and group Ⅱ were added to feed the active egg white protein and Saccharomyces cerevisiae for 10 days， and 28 day old piglets were weaned. On 23， 28， 35 and 42 day old， a piglet randomly selected from each replication was slaughtered， both ends of colon were ligated quickly and the colonic digesta were collected aseptically to determine colonic SCFA by gas chromatography. The results showed that: (1) On 28 day old， compared with those in group Ⅲ piglets， the colonic acetic acid(P<001)， propionic acid(P<005)， butyric acid(P<005) and total SCFA(P<001) concentrations in group Ⅰ piglets increased， isobutyric acid concentration in group Ⅰ piglets decreased(P<001); compared with those in group Ⅱ piglets， the colonic acetic acid(P<001)， isobutyric acid(P<001) and total SCFA(P<001) concentrations in group Ⅰ piglets increased; (2) On 42 day old， compared with those in group Ⅱ and group Ⅲ piglets， the colonic acetic acid(P<005)， propionic acid(P<005)， butyric acid(P<005)， isovaleric acid(P<005) and total SCFA(P<005) concentrations in group Ⅰ piglets increased; (3) With the increase of piglet age， total SCFA showed a increasing tendency in every group， but group Ⅰ was most obvious. It followed that active egg white protein could improve colonic SCFA concentration of weaned piglets．