| 一个水稻矮秆窄叶突变体的鉴定和基因定位 |
| |
| 引用本文： | 刘继云,叶胜海,李小华,赵小燕,尚海漩,纪现军,邓晓梅,陈萍萍,金庆生,张小明. 一个水稻矮秆窄叶突变体的鉴定和基因定位[J]. 浙江农业学报, 2014, 26(1): 0 |
| |
| 作者姓名： | 刘继云 叶胜海 李小华 赵小燕 尚海漩 纪现军 邓晓梅 陈萍萍 金庆生 张小明 |
| |
| 作者单位： | 1.浙江师范大学 化学与生命科学学院，浙江 金华 321004；2浙江省农业科学院 作物与核技术利用研究所，浙江 杭州 310021；3 杭州师范大学 生命与环境科学学院，浙江 杭州 310036 |
| |
| 基金项目： | 浙江省科技厅项目(2012C12901-2);国家自然科学基金项目(31071207) |
| |
| 摘 要： | 常规粳稻秀水09经过甲基磺酸乙酯（EMS）诱变处理，获得了一个矮秆窄叶突变体dnl1（dwarf and narrow leaf 1），经多代自交性状稳定。dnl1突变体与野生型相比，具有植株矮、叶片窄、分蘖强的特点。遗传分析表明dnl1突变性状受1对隐性核基因控制。通过图位克隆的方法，利用SSR分子标记将突变基因dnl1定位在第4号染色体SSR标记RM17478与RM17488之间约260 kb范围内。测序比对分析表明Dnl1可能为Nal1基因.
|
| 关 键 词： | 水稻 矮秆窄叶 图位克隆 分子定位 |
Analysis and mapping of a dwarf and narrow leaf rice mutant |
| |
| LIU Ji|yun,YE Sheng|hai,LI Xiao|hua,ZHAO Xiao|yan,SHANG Hai|xuan,JI Xian|jun,DENG Xiao|mei,CHEN Ping|ping,JIN Qing|sheng,ZHANG Xiao|ming. Analysis and mapping of a dwarf and narrow leaf rice mutant[J]. Acta Agriculturae Zhejiangensis, 2014, 26(1): 0 |
| |
| Authors: | LIU Ji|yun YE Sheng|hai LI Xiao|hua ZHAO Xiao|yan SHANG Hai|xuan JI Xian|jun DENG Xiao|mei CHEN Ping|ping JIN Qing|sheng ZHANG Xiao|ming |
| |
| Affiliation: | 1College of Chemistry and Life Science， Zhejiang Normal University， Jinhua 321004， China；2 Institute of Crop and Nuclear Technology Utilization，Zhejiang Academy of Agricultural Sciences， Hangzhou 310021，China；3 College of Life and Environment Sciences， Hangzhou Normal University， Hangzhou 310036，China） |
| |
| Abstract: | A dwarf and narrow leaf （dnl1） mutant was isolated by ethylmethylsulfonate （EMS） treating for a japonica rice variety Xiushui 09 dry seeds，it could be inherited stably after several selfing. Compared with its wild type Xiushui 09， the mutant shows dwarf， narrow leaf and more tillers. Genetic analysis showed that the traits of the dnl1 mutants were controlled by one pair of recessive nuclear gene. With map|based cloning approach， Dnl1 gene was mapped between SSR marker RM17478 and RM17488 on the chromosome 4， a 260 kb region. Sequencing and blast analysis indicated that Dnl1 is an allele to the gene Nal1. |
| |
| Keywords: | rice dwarf and narrow leaf map|based cloning molecular mapping |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《浙江农业学报》浏览原始摘要信息 |
|
点击此处可从《浙江农业学报》下载全文 |
