抗禽大肠杆菌中药的体外抑菌方法和效果的研究

为研究金银花、大青叶等10味中药对禽类大肠杆菌的体外抗菌效果，使用纸片法、打孔法同时对10种中药水提物进行体外抑菌试验，通过抑菌结果筛选较优抑菌试验方法，并测定抑菌效果较好中药的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明，两种方法检测的药物抑菌圈大小除甘草、重楼外，均呈现显著性差异(P≤005)，且其中的金银花、黄柏、贯众、黄连四味药抑菌结果差异极显著(P＜001)，说明纸片法效果更佳；10味中药中金银花、黄连的抑菌圈直径最大，分别为(1510±001)，(1602±001) mm，呈高度敏感反应；二者的MIC分别是625，3125 mg·mL-1，MBC为125，625 mg·mL-1。试验得出较好的体外抑菌试验方法为纸片法，10种抗禽大肠杆菌中药中以金银花、黄连效果最佳。

Study on external bacteriostasis tests of Chinese herbal medicine against avian E.coli and their antibacterial effects

In order to study the inhibitory effect of 10 kinds of Chinese herbal medicine including honeysuckle， folium isatidis against avian E. coli in vitro， an external bacteriostasis experiment was conducted for the 10 kinds of Chinese herbal medicine by using concurrently the Kirby Bauer method and punch method， then a better method for inhibitory test was selected， and the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of the highly sensitive Chinese herbal medicine against avian E. coli was determined. The results showed that bacteriostasis circle size of two methods showed significant differences (P≤005) except for the licorice， heavy flooror， and showed extremely significant differences (P＜001)for honeysuckle， cortex phellodendri， cyrtomium rhizome， rhizoma coptidis among them，which indicated that the Kirby Bauer method was better. The bacteriostasis diameter of honeysuckle and coptis were larger among the10 kinds of Chinese herbal medicine， which were (1510±001) mm， (1602±001) mm respectively， belonging to the high sensitivity. The MIC of the honeysuckle and coptis were 625 mg·mL-1， 3125 mg·mL-1， MBC were 125 mg·mL-1， 625 mg·mL-1. The Kirby Bauer method was the best in vitro bacteriostasis test method， the best bacteriostasis effect of 10 kinds of Chinese herbal medicine against avian E.coli were honeysuckle and coptis．