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| 猪肉和鸡肉中脱氧雪腐镰刀菌烯醇和T-2毒素酶联免疫吸附检测方法的建立 | |
| 引用本文： | 宋晓丽,刘梅轩,罗湘蜀,李成龙,杨术鹏,王战辉,张素霞. 猪肉和鸡肉中脱氧雪腐镰刀菌烯醇和T-2毒素酶联免疫吸附检测方法的建立[J]. 中国畜牧兽医, 2015, 42(3): 525-530. DOI: 10.11843/j.issn.1671-7236.2015.03.003 |
| 作者姓名： | 宋晓丽 刘梅轩 罗湘蜀 李成龙 杨术鹏 王战辉 张素霞 |
| 作者单位： | 1. 中国农业大学动物医学院, 北京 100193; 2. 国家兽药残留基准实验室, 北京 100193 |
| 基金项目： | “973”国家重点基础研究发展计划课题(No.2009CB118801)“受试化合物在畜禽体内的代谢研究” |
| 摘 要： | 为保障动物源性食品安全,本研究建立了分别检测猪肉和鸡肉中脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)和T-2毒素残留的间接竞争酶联免疫吸附法.结果显示,该方法在猪肉和鸡肉样本中DON的检测限分别为34.9和43.5 μg/kg,添加回收率为72.7%~97.1%,变异系数小于8.7%;T-2毒素的检测限分别为33.7和28.7 μg/kg,添加回收率为72.1%~95.0%,变异系数小于11.3%.该方法灵敏度高、准确、简便,适用于猪肉和鸡肉中DON和T-2毒素残留的快速检测. |
| 关 键 词： | 酶联免疫吸附法(ELISA) 脱氧雪腐镰刀菌烯醇(DON) T-2毒素 猪肉 鸡肉 |
| 收稿时间： | 2014-10-14 |
Development of an Enzyme-linked Immunosorbent Assay for the Detection of Deoxynivalenol and T-2 Toxin in Pork and Chicken |
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| SONG Xiao-li;LIU Mei-xuan;LUO Xiang-shu;LI Cheng-long;YANG Shu-peng;WANG Zhan-hui;ZHANG Su-xia. Development of an Enzyme-linked Immunosorbent Assay for the Detection of Deoxynivalenol and T-2 Toxin in Pork and Chicken[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(3): 525-530. DOI: 10.11843/j.issn.1671-7236.2015.03.003 | |
| Authors: | SONG Xiao-li LIU Mei-xuan LUO Xiang-shu LI Cheng-long YANG Shu-peng WANG Zhan-hui ZHANG Su-xia |
| Affiliation: | 1. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; 2. National Reference Laboratory for Veterinary Drug Residue, Beijing 100193, China |
| Abstract: | To protect animal-origin foods,an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to detect deoxynivalenol (DON) and T-2 toxin in pork and chicken,respectively.The results showed that the limit of detection (LOD) of DON in pork and chicken were 34.9 and 43.5 μg/kg,respectively,and the recoveries ranged from 72.7% to 97.1% with the coefficients of variation (CV) lower than 8.7%.Besides,the LOD of T-2 toxin in pork and chicken were 33.7 and 28.7 μg/kg,respectively,and the recoveries ranged from 72.1% to 95.0% with CV less than 11.3%.The developed method had the advantage of being sensitive and accurate,and was suitable for the rapid detection of DON and T-2 residue in pork and chicken. |
| Keywords: | enzyme-linked immunosorbent assay (ELISA) deoxynivalenol (DON) T-2 toxin pork chicken |
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