| Asia1型口蹄疫病毒基因组的测序分析 |
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| 引用本文： | 王琳萱,陈思敏,张印红,孙东杰.Asia1型口蹄疫病毒基因组的测序分析[J].浙江农业学报,2014,26(2):0. |
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| 作者姓名： | 王琳萱 陈思敏 张印红 孙东杰 |
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| 作者单位： | 1.兰州大学 公共卫生学院营养与食品卫生研究所，甘肃 兰州 730000；2中国农业科学院 兰州兽医研究所/家畜疫病病原生物学国家重点实验室，甘肃 兰州 730046 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项基金项目(1610322013023) |
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| 摘 要： | 对中国口蹄疫病毒Asia1/JS/2005毒株的全基因组进行了分段扩增并测序。结果表明，该毒株的基因组全长为8 174 nt，其中 5’UTR 为1 092 nt；ORF为6 990 nt；3’UTR为92 nt。利用DNAstar软件对该毒株和Asia1型其他24毒株基因组序列进行同源性分析表明，该毒株的非结构蛋白和5’UTR比较保守。研究还发现了可能与生物学功能相关的新的保守和变异区域。系统树分析表明Asia1/JS/2005， Asia1/1/YZ/2006，Asia1/WHN/2006，Asia1/HN/2006和Asia1/YS/2005属于同一分支，表明这些毒株间有着紧密的遗传关系，其中与其亲缘关系最为密切的是Asia1/YS/2005。
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| 关 键 词： | 口蹄疫病毒 Asia1/JS/2005 基因组 |
Genome sequencing and analysis in Asia1 serotype strain of foot and mouth disease virus |
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| WANG Lin xuan,CHEN Si min,ZHANG Yin hong,SUN Dong jie.Genome sequencing and analysis in Asia1 serotype strain of foot and mouth disease virus[J].Acta Agriculturae Zhejiangensis,2014,26(2):0. |
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| Authors: | WANG Lin xuan CHEN Si min ZHANG Yin hong SUN Dong jie |
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| Institution: | 1Nutrition and Food Hygiene Research Institute， College of Public Hygiene， Lanzhou University， Lanzhou 730000， China; 2 Veterinary Research Institute， Chinese Academy of Agricultural Sciences/State Key Laboratory of Veterinary Etiological Biology， Lanzhou 730046， China |
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| Abstract: | In this study， the whole genome of foot and mouth disease virus (FMDV) vaccine strain Asia1/JS/2005 in China was amplified and sequenced. The results showed that the complete genome is 8 174 nt， including a 1 092 nt 5’ untranslated region (UTR)， a 6 990 nt open reading frame and a 92 nt 3’UTR. By using the DNAstar software， homology analysis of genome sequences of strain Asia1/JS/2005 and other 24 Asia1 strains were performed. The results indicated that non structural proteins and 5’UTR were more conserved. The data revealed novel highly conserved and variable genomic regions， which was likely associated with biological function. Phylogenetic tree revealed that Asia1/JS/2005 is closely linked with Asia1/1/YZ/2006， Asia1/WHN/2006， Asia1/HN/2006 and Asia1/YS/2005， which suggested that they were genetically associated and Asia1/YS/05 was the most closely related strain with Asia1/JS/2005． |
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| Keywords: | foot and mouth disease virus Asia1/JS/2005 genome |
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