| 颈盲蝽取食对薇甘菊叶片防御性酶活性的影响 |
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| 引用本文： | 季梅,泽桑梓,赵宁,杨斌.颈盲蝽取食对薇甘菊叶片防御性酶活性的影响[J].浙江农业学报,2014,26(3):0. |
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| 作者姓名： | 季梅 泽桑梓 赵宁 杨斌 |
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| 作者单位： | 1.云南省林业科学院，云南省森林植物培育与开发利用重点实验室，云南 昆明 650201；2云南林业职业技术学院， 云南 昆明 650224；3西南林业大学，云南 昆明 650224 |
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| 基金项目： | 国家林业公益性行业科研专项项目(201204518);云南省社会发展科技计划项目(2012CH001);云南省应用基础研究面上项目(2010CD131) |
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| 摘 要： | 为探明颈盲蝽Pachypeltis sp.取食对薇甘菊叶片内主要防御酶活性的影响，以薇甘菊为材料，室内条件下测定颈盲蝽取食12，24，48，96 h后薇甘菊叶片中过氧化物酶(POD)、多酚氧化酶(PPO)及苯丙氨酸解氨酶(PAL)的活性。结果表明，颈盲蝽取食后薇甘菊叶片中的POD和PAL的活性下降，PPO活性提高。取食48 h后，处理组叶片中POD和PAL活性分别下降了5191%和7500%，而相应的对照组叶片则分别下降了3234%和4986%；处理组和对照组叶片PPO活性分别上升了37173%和12113%。
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| 关 键 词： | 薇甘菊 颈盲蝽 取食 防御酶活性 |
Effects of Pachypeltis sp. feeding on defense enzyme activities in Mikania micrantha leaves |
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| JI Mei,ZE Sang zi,ZHAO Ning,YANG Bin.Effects of Pachypeltis sp. feeding on defense enzyme activities in Mikania micrantha leaves[J].Acta Agriculturae Zhejiangensis,2014,26(3):0. |
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| Authors: | JI Mei ZE Sang zi ZHAO Ning YANG Bin |
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| Institution: | 1Yunnan Academy of Forestry， Yunnan Provincial Key Laboratory for Cultivation and Exploitation of Forest Plants， Kunming 650201， China; 2Yunnan Forestry Technological College， Kunming 650224， China; 3Southwest Forestry University， Kunming 650224， China |
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| Abstract: | To study the effects of feeding by Pachypeltis sp. on the defense enzymes in Mikania micrantha， activities of three enzymes， peroxidase (POD)， polyphenol oxidase (PPO) and phenylalanine aminolyase (PAL)， were evaluated in M. micrantha leaves at time points of 12h， 24h， 48h and 96h post feeding under laboratory conditions. The results showed that activities of POD and PAL decreased while PPO increased. At 48 h after feeding， POD and PAL activities of the treatment group decreased by 5191% and 7500% respectively， while that of the control group decreased by 3234% and 4986%. PPO activity of the treatment group increased by 37173%， while that of the control group increased by 12113%． |
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| Keywords: | Mikania micrantha Pachypeltis sp feeding defense enzymes |
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