木耳总RNA提取方法研究

为了获得木耳总RNA理想的提取方法，为后续的分子生物学研究打下基础，以木耳菌丝为试验材料，采用了CTAB法、RNA prep pure Plant Kit试剂盒和 Trizol法3种方法提取木耳总RNA。结果表明3种方法均能从木耳菌丝中提取分离到总RNA，然而Trizol 法能提取到纯度较高、完整性较好的总RNA，28S及18S带型清晰无弥散，A260/A280值为191，RNA 产率为68264 μg·g-1，提取的总 RNA 适用于 RT PCR等分子生物学试验研究。

Study on methods for RNA extraction from Auricularia auricular

The purity and integrity of total RNA are essential for molecular biology experiments of Auricularia auricular. In order to obtain an ideal total RNA extraction method， total RNA of Auricularia auricular was extracted with 3 methods， including Trizol method， CTAB method and RNA prep pure Plant Kit method. The results showed that all these methods can extract total RNA from Auricularia auricular; however， the Trizol method was the best with high integrity and purity. The band of 28S was twice wider than that of 18S. The RNA yield was 68264 μg·g-1 and the RNA sample could be used for RT PCR.