花生序列相关扩增多态性(SRAP)标记的研究

研究了分属3个市场型的10个中国花生栽培种的序列相关扩增多态性（SRAP）。结果表明，SARP技术可以揭示花生栽培种的遗传差异。在所试验的51对SRAP引物中，2对只获得了单态性条带，其余49对总共产生了1087条带，其中503条（46．27％）为多态性带，每对引物组合平均获得22．18条总带、10．26条多态性带。在这49对引物组合中，总带数和多态性条带的数目分别为7～63和2～32条。 10个花生栽培种间的简单匹配相似系数为0．7712～0．9250不等，根据SRAP指纹图谱进行聚类分析可将这10个品种分为4类。另外一项采用作图亲本24-3和B4的研究中，40对SRAP引物共计获得了160条多态性带。本研究为花生品种鉴定和遗传研究提供了新的DNA分子标记技术手段。

The Sequence-related Amplification Polymorphisms in Cultivated Peanut (Arachis hypogaea L.)

SRAP assay was conducted using 10 peanut cultivars from China of 3 market types and 51 SRAP primer combinations to evaluate the utility of SARP in peanut. Of the primer pairs tested, 2 produced monomorphic bands only, and 49 totally produced 1087 bands, among which 503 (46.27%) were polymorphic, with an average of 10.26 polymorphic bands and 22.18 bands per primer combination. The total number of bands and polymorphic bands in the 49 primer combinations ranged from 7~63 and 2~32 respectively. The simple matching coefficient of the peanut varieties ranged from 0.7712 to 0.9250. The varieties may be grouped into 4 categories based on cluster analysis of the SRAP fingerprinting data. In a separate study on SARP using 40 primer pairs, 160 polymorphic bands were detected in 24-3 and B4,the parental genotypes for mapping. This study provided an additional marker system for peanut variety identification and genetic research.