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| 虾夷扇贝毒素对ICR小鼠小脑浦肯野细胞毒性作用的初步研究 | |
| 引用本文： | 庞敏,高春蕾,王宗灵,唐学玺.虾夷扇贝毒素对ICR小鼠小脑浦肯野细胞毒性作用的初步研究[J].水产学报,2010,34(3):435-440. |
| 作者姓名： | 庞敏 高春蕾 王宗灵 唐学玺 |
| 作者单位： | 1. 中国海洋大学海洋生命学院,山东,青岛,266003;国家海洋局第一海洋研究所,山东,青岛,266061 2. 国家海洋局第一海洋研究所,山东,青岛,266061 3. 中国海洋大学海洋生命学院,山东,青岛,266003 |
| 基金项目： | 科技部国际合作项目(2007DFA30710); 国家海洋局青年基金(2008108) |
| 摘 要： | 以雌性ICR小鼠为研究对象,尾静脉注射虾夷扇贝毒素24h后处死,通过脑组织切片,苏木精伊红染色、甲苯胺蓝染色以及钙结合蛋白S-100免疫染色,研究了虾夷扇贝毒素对小鼠浦肯野细胞的毒性作用。结果表明,尾静脉注射虾夷扇贝毒素的小鼠小脑浦肯野细胞细胞质有明显变化,同时在光学显微镜下观察到细胞核有收缩而用钙离子结合蛋白S-100的免疫荧光染色结果表明,虾夷扇贝毒素作用后,小鼠浦肯野细胞的S-100蛋白表达增多,而其他组织则没有明显变化。浦肯野细胞形态学以及细胞内钙离子结合蛋白的变化说明虾夷扇贝毒素可能对神经细胞有毒性作用。 |
| 关 键 词： | 虾夷扇贝毒素 浦肯野细胞 细胞毒性 钙离子结合蛋白 |
| 收稿时间： | 2009/5/19 0:00:00 |
| 修稿时间： | 2009/8/31 0:00:00 |
Preliminary studies on toxicity induced by yessotoxin(YTX) on Purkinje cells from the cerebellum of ICR mice |
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| PANG,GAO Chunlei,WANG Zonglin and TANG Xuexi.Preliminary studies on toxicity induced by yessotoxin(YTX) on Purkinje cells from the cerebellum of ICR mice[J].Journal of Fisheries of China,2010,34(3):435-440. | |
| Authors: | PANG GAO Chunlei WANG Zonglin and TANG Xuexi |
| Institution: | PANG Min 1,2,GAO Chun-lei2,WANG Zong-ling 1,TANG Xue-xi1 (1.College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China,2.The First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China) |
| Abstract: | Yessotoxin(YTX) and its analogues are disulfated polyether compounds which have been concerned more in seafood quality monitoring and shellfish industries recently. Many studies on the toxicity of YTXs had been carried out,but the results showed apparent discrepancies. Just these discrepancies have encouraged many researchers to study the mechanism by which YTX exerts its action,such as what the target tissues are,what the pattern of the mode and precise mechanism of action are. However,the process still re... |
| Keywords: | yessotoxins Purkinje cells cytotoxic calcium binding protein |
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