Leaf proteins as markers useful in the genetic improvement of coconut palms |
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Authors: | Rolando Cardeña Carlos Oropeza Daniel Zizumbo |
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Institution: | (1) Centro de Investigación Científica de Yucatán, A.C., A.P. 87, Mérida, 97310 Yucatán, México |
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Abstract: | There is a need to identify genetic markers that can assist coconut (Cocos nucifera L.) breeding programs. With that objective,
electrophoretic patterns of leaf peroxidases, endopeptidases and coomassie blue stained proteins were analysed in four cultivars
(‘West African Tall’, ‘Rennell Tall’, ‘Malayan Yellow Dwarf’, ‘Cameroon Red Dwarf’), and in the hybrids PB121 (‘Malayan Yellow
Dwarf’ × ‘West African Tall’) and PB111 (‘Cameroon Red Dwarf’ × ‘West African Tall’). The polymorphisms detected fit the expression
of two alleles of a dimeric peroxidase, two alleles of a monomeric endopeptidase, and a pair of active and null alleles of
a coomassie blue stained protein. Four distinctive genotypes were identified. One for each of the tall cultivars, another
for both of the dwarf cultivars, and the last for both of the hybrids. Applications of the markers in breeding programs, and
in research concerning reproductive biology and phylogeny, are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Cocos nucifera coconut isozymes cultivar differentiation enzyme extraction variation |
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