| 免疫亲和层析法分离鸡补体C3d蛋白研究 |
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| 引用本文： | 马雪云,韩建秋,高淑霞.免疫亲和层析法分离鸡补体C3d蛋白研究[J].西北农林科技大学学报(社会科学版),2006,34(5):55-58. |
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| 作者姓名： | 马雪云 韩建秋 高淑霞 |
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| 作者单位： | 1. 山东农业大学,动物科技学院,山东,泰安,271018
2. 山东省枣庄市,园林管理处,山东,枣庄,277102 |
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| 摘 要： | 利用抗鸡C3 21肽抗体制备免疫亲和层析柱,对鸡补体C3d进行了分离,并采用间接酶联免疫吸附实验(间接ELISA),对洗脱液中的C3d组分进行了检测,以SDS-PAGE电泳方法对分离到的C3d进行了检测。结果表明,利用亲和柱层析能够分离得到纯度较高的C3d蛋白,为鸡血清中C3d的提取纯化提供了较为简便的方法。
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| 关 键 词： | 鸡补体C3d 免疫亲和层析 间接ELISA |
| 文章编号： | 1671-9387(2006)05-0055-04 |
| 收稿时间： | 2005-09-05 |
| 修稿时间： | 2005年9月5日 |
Study on isolating complement C3d from the chicken serum by the method of immuno affinity chromatography |
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| MA Xue-yun,HAN Jian-qiu,GAO Shu-xia,NIU Zhong-xiang.Study on isolating complement C3d from the chicken serum by the method of immuno affinity chromatography[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(5):55-58. |
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| Authors: | MA Xue-yun HAN Jian-qiu GAO Shu-xia NIU Zhong-xiang |
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| Institution: | (College of Animal Science and Technology,Shandong Agricultural University ,Tai’an,Shandong 271018,China; Zaozhuang Garde Manage Bureau Shandong Province, Zaozhuang ,Shandong 277102,China) |
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| Abstract: | Using the antibody of 21 peptide from the complement C3's a chian of chicken,the affinity chromatography column was fabricated and the complement C3d was isolated. With the method of indirect ELISA,the C3d portion in the solution was tested,and the isolated C3d was examined with the method of SDS-PAGE. It showed that by using the method of affinite chromatography,the pure C3d protein can be obtained. The method provides a simple way to purify the chicken C3d. |
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| Keywords: | complement C3d immunol affinity chromatography indirect ELISA |
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