甜瓜枯萎病拮抗内生细菌筛选研究

从长势健壮的黄瓜、南瓜和甜瓜苗的根部及茎部分离获得294株内生细菌,与枯萎尖孢镰孢菌进行对峙培养,获得具有抑茵活性细菌18株,占总茵株数的6.1%.再利用生长速率法、孢子萌发法对其进行体外生物活性检测,结果表明,H-2、H-4、H-6、N-7、T-16、T-18 等 6 个菌株的发酵滤液对枯萎镰孢菌的桔抗作用较强,但H-1与H-4对种子萌芽有强烈的抑制作用.H-5、H-6 和 N8 等菌株对甜瓜幼苗具有促生作用,对甜瓜枯萎病的防效分别为75.6%、60.1%和66.7%.H-5 菌株对甜瓜枯萎病可能具有潜在的生防功能.

Selection of Endophytic Bacteria for Suppression of Melon Wilt Caused by Fusarium oxysporum

Two hundreds and ninety-four strains of endophytic bacteria were isolated from roots and stems of healthy cucumber,pumpkin and melon seedlings.By duel test of in vitro screening,about 6.1% of the total strains screened,18 antagonistic strains against Fusarium oxysporum f.sp.melonis(Leach) Syn were gotten.Among the 18 strains,6 strains(H-2,H-4,H-6,N-7, T-16,and T-18) had been proved that their fermented solution inhibited significantly Fusarium oxysporum by using in vitro dilution plate technique test and spore germination test,2 strains(H-1 and H-4) inhibited melon seed germination.The growth-promoting effects on melon seedling,and control effects against melon wilt of the 18 strains were studied.The results showed that control efficacies of 3 strains,H-5,H-6 and N-8,were 75.6%,60.1% and 66.7% respectively,meanwhile,their growth promoting effect were found also.H-5 was able to be a bio-control agent to control the melon wilt Fusarium oxysporum.