| 鸭肝炎病毒单克隆抗体的制备 |
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| 引用本文： | 潘朋朋,文力正,单振振,丁毅,袁宝,任文陟,李维娜,李晓叶.鸭肝炎病毒单克隆抗体的制备[J].中国农学通报,2011,27(1):415-418. |
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| 作者姓名： | 潘朋朋 文力正 单振振 丁毅 袁宝 任文陟 李维娜 李晓叶 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,长春,130062
2. 吉林大学实验动物中心,长春,130062 |
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| 基金项目： | 吉林省科技平台建设项目"吉林省实验动物质量检测中心平台建设" |
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| 摘 要： | 本实验利用弗式完全佐剂和弗式不完全佐剂，乳化病毒，制备免疫抗原，对BALB/c小鼠进行三次免疫。将免疫效价达到1：10000以上的免疫小鼠脾细胞与SP2/0细胞进行融合，通过间接ELISA方法对阳性杂交瘤细胞株进行筛选，并通过有限稀释法将呈强阳性的杂交瘤细胞亚克隆3～4次，直到杂交瘤细胞阳性率达到100%。得到阳性株命名为2E3，对阳性株细胞进行扩大培养，并将细胞注入腹腔，提取腹水，测定细胞上清及腹水的效价,分别为10×25，10×26。利用亚类鉴定试剂盒测定单抗的亚类。鉴定结果为IgG1型。
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| 关 键 词： | 生物表面活性剂 生物表面活性剂 生物修复 石油污染 土壤 微生物 |
| 收稿时间： | 2010/6/25 0:00:00 |
| 修稿时间： | 8/2/2010 12:00:00 AM |
Preparation of Monoclonal Antibody against Duck Hepatitis Virus |
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| Pan Pengpeng,Wen Lizheng,Shan Zhenzhen,Ding Yi,Yuan Bao,Ren Wenzhi,Li Weina,Li Xiaoye.Preparation of Monoclonal Antibody against Duck Hepatitis Virus[J].Chinese Agricultural Science Bulletin,2011,27(1):415-418. |
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| Authors: | Pan Pengpeng Wen Lizheng Shan Zhenzhen Ding Yi Yuan Bao Ren Wenzhi Li Weina Li Xiaoye |
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| Institution: | Pan Pengpeng 1,Wen Lizheng 2,Shan Zhenzhen 1,Ding Yi 1,Yuan Bao 2,Ren Wenzhi 2,Li Weina 1,Li Xiaoye 1 ( 1 College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,2 Laboratory Animal Center,Changchun 130062) |
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| Abstract: | In order to make DHV antigens,the experiment utilize IFA and CFA. The BALB/c mice were immunized with DHV for three times. The spleen ’ s B lymphocyte of the mice should be fused with SP2/0 plasmacytoma cells until the immunopotency was up to 1：10000. The masccline hybridoma cells were screened by indirect enzyme-link immunosorbent assay. Hybridomas whose supernatants show stronger positive were subcloned by limited dilution methods for 3~4 times, until the positive rate come up to 100%. It was named 2E3. The immunopotency of ascites and supernatant was 10×2 5 ,100×2 6. . And the subtype of 2E3 was IgG l . |
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| Keywords: | ELISA |
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