| 抗草甘膦转基因大豆对根际土壤细菌多样性的影响 |
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| 引用本文： | 李刚 赵建宁 杨殿林. 抗草甘膦转基因大豆对根际土壤细菌多样性的影响[J]. 中国农学通报, 2011, 27(1): 100-104 |
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| 作者姓名： | 李刚 赵建宁 杨殿林 |
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| 作者单位： | 农业部环境保护科研监测所,农业部转基因生物生态环境安全监督检验测试中心,天津,300191 |
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| 基金项目： | 转基因生物新品种培育重大专项课题，中央级公益性科研院所基本科研业务费专项资金 |
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| 摘 要： | 采用DGGE-cloning测序技术研究抗草甘膦转基因大豆在生长期内对根际土壤细菌多样性的影响。结果分析表明，不同生长期内抗草甘膦转基因大豆与非转基因大豆根际土壤细菌16S rDNA DGGE指纹图谱谱形相似，仅在出苗期和鼓粒期出现两条差异带，分别属于芽单胞菌门（Uncultured Gemmatimonadetes bacterium clone，缺失）和壁厚菌门（Uncultured Firmicutes bacterium clone GASP-KC3W1_H04，增加）；不同生长期内抗草甘膦转基因大豆与非转基因大豆根际土壤细菌16S rDNA DGGE指纹图谱的多样性指数和均匀度指数无显著差异。因此，抗草甘膦转基因大豆对土壤细菌多样性无显著影响。
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| 关 键 词： | ACCase ACCase accB 载体构建 原核表达 IPTG诱导 |
| 收稿时间： | 2010-07-02 |
| 修稿时间： | 2010-07-28 |
Effects of Glyphosate Resistant Transgenic Soybean on Bacterial Diversity in Rhizospheric Soil |
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| Li Gang,Zhao Jianning,Yang Dianlin. Effects of Glyphosate Resistant Transgenic Soybean on Bacterial Diversity in Rhizospheric Soil[J]. Chinese Agricultural Science Bulletin, 2011, 27(1): 100-104 |
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| Authors: | Li Gang Zhao Jianning Yang Dianlin |
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| Affiliation: | Li Gang,Zhao Jianning,Yang Dianlin ( Ecological Environment Safety Supervision,Inspection and Testing Center of GMOs,Agro-Environmental Protection Institute,Ministry of Agriculture,Tianjin 300191) |
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| Abstract: | DGGE-cloning sequencing method was chosen to study the effects of the planting of glyphosate resistant transgenic soybean (Roundup Reandy Soybean, RRS) on bacterial diversity in the rhizospheric soil during the growing period. The results showed that the rhizospheric bacterial 16S rDNA DGGE fingerprintings between glyphosate resistant transgenic soybean and non-transgenic soybean were similar within different growing stages, with only two different bands in the stages of emerging and feed filling, the two b... |
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| Keywords: | glyphosate resistant transgenic soybean rhizospheric soil bacterial diversity DGGE-cloning sequencing method |
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