| 以小鼠骨髓间充质干细胞为饲养层分离培养胚胎干细胞的研究 |
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| 引用本文： | 杨恕玲,单守明,何生虎.以小鼠骨髓间充质干细胞为饲养层分离培养胚胎干细胞的研究[J].中国农学通报,2011,27(1):370-373. |
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| 作者姓名： | 杨恕玲 单守明 何生虎 |
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| 作者单位： | 宁夏大学农学院,银川,750021 |
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| 基金项目： | 宁夏回族自治区科技兴农计划项目"奶牛疾病防控技术研究" |
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| 摘 要： | 【研究目的】为了探求一种更简单有效地分离培养昆明小鼠胚胎干细胞的饲养层培养体系；【研究方法】将昆明小鼠3.5d的胚胎，接种于昆明小鼠骨髓间充质干细胞的饲养层培养体系，来分离培养昆明小鼠的胚胎干细胞。【结果】发现在骨髓间充质干细胞的饲养层培养体系上，获得了典型的ES细胞样集落，碱性磷酸酶染色呈阳性，且具有分化的潜能。【结论】小鼠骨髓间充质干细胞可以作为饲养层来分离培养ES细胞，并且能保持ES细胞的体外未分化状态。
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| 关 键 词： | 小白菜 小白菜 硝酸盐 亚硝酸盐 基因型差异 吸收动力学 |
| 收稿时间： | 6/1/2010 12:00:00 AM |
| 修稿时间： | 2010/7/21 0:00:00 |
The Study of Using Bone Marrow Mesenchymal Stem Cells of Mouse as Feeder Layer Cells in Cultivation of Embryonic Stem Cells |
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| Yang Shuling,Shan Shouming,He Shenghu.The Study of Using Bone Marrow Mesenchymal Stem Cells of Mouse as Feeder Layer Cells in Cultivation of Embryonic Stem Cells[J].Chinese Agricultural Science Bulletin,2011,27(1):370-373. |
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| Authors: | Yang Shuling Shan Shouming He Shenghu |
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| Institution: | Yang Shuling,Shan Shouming,He Shenghu (College of Agronomy,Ningxia University,Yinchuan 750021 ) |
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| Abstract: | In order to find the more simple method of preparition for the layer feeder cells, the blastocytes of 3.5 days embryo of Kunming mouse cultured on bone marrow mesenchymal stem cells were used to isolate and culture the ES cells. The colonies of ES cells were harvested to passage. The growth of stem cells identified was observed , and their biological vitality was determined by alkaline phosphatase activity assay and cell differentiation examination. The blastocytes of 3.5 days embryo from Km mouse could be ... |
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| Keywords: | bone marrow mesenchymal stem cells embryonic stem cell cell culture |
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