| Preliminary Studies on Establishment of Genetic Transformation System in Loblolly Pine |
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| 引用本文： | 唐巍,田颖川,欧阳藩. Preliminary Studies on Establishment of Genetic Transformation System in Loblolly Pine[J]. 林业研究, 1997, 0(4) |
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| 作者姓名： | 唐巍 田颖川 欧阳藩 |
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| 作者单位： | Tang Wei(State key Laboratory of Biochemical Engineering. Institute of Chemical Metallurgy，Chinese Academy of Sciences，Beijing 100080. P. R. China)Tian Yingchuan(Institute of Microbiology. Chinese Academy of Sciences. Beijing 100080)Ouyang Fan(State key |
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| 摘 要： | Introducti0nt,ollilcrsareec0n0l11icall}impo11a11tl0resttree`orlLl-tvide.beingusedtbrs0iIc0nservatio11-el1vir()11111e11lprotectiol1,`ood.prodtlctiOI1.andasavariet}ofhIIiId-ingandil1dLlstr}'Inaterial.ForcOI1itbrgel1cticiI11-provel11el1tplan-classicalbreed…
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Preliminary Studies on Establishment Genetic Transformation System in Loblolly Pine |
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| Tang Wei. Preliminary Studies on Establishment Genetic Transformation System in Loblolly Pine[J]. Journal of Forestry Research, 1997, 0(4) |
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| Authors: | Tang Wei |
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| Abstract: | A transformation system was established for loblolly pine (Pimus taeda L ) mature zygotic embryos using Agrobacterium tumefaciens.The gene coding for the glucuronidase (GUS) gene was introduced into loblolly pine tissues andits transient expression was detected with histochemical staining. The influences of different genotypes. Agrobacterium concentrations. and cocultivation time on GUS expression and Kanamycin resistant callus and shoot regeheration were investigated. The results showed that the highest `GUS expression frequency (1 6.3%) and shoot regencration frequency(7.8%) wereobtained from genotype 9-1003 with, Agrobactemm concentration decreased 9 times and cocultivation time of 56 hours.respectively GUS expression was, obtained in all genotypes tested The successtul expression of the GUS gene in differentgenotypes suggested that it will be a useful transformation system for loblolly pine |
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| Keywords: | Pinus taeda L. Genetic transformation Agrobacterium tumefaciens |
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