猪源支气管败血波氏杆菌河南株的分离鉴定

从河南省多个养猪场采集126份有呼吸道症状猪的肺脏组织样品或鼻拭子,进行支气管败血波氏杆菌的分离鉴定。根据细菌培养特性、革兰氏染色镜检、生化试验和PCR鉴定,确认分离出11株支气管败血波氏杆菌(Bordetella bronchisepti-ca,Bb)。对Bb最重要的3种毒力因子fha、prn、dnt基因进行PCR检测,除2株为prn基因阴性外,其它PCR结果均为阳性。红细胞凝集试验显示各菌株均能不同程度的凝集猪和羊的红细胞。乳鼠皮肤坏死试验表明,各菌株均能不同程度的造成乳鼠皮肤坏死。通过小鼠毒力试验,筛选到4株强毒菌株(HN0710、HN0806、HN0922、HN0827)。

The Isolation and Identification of Swine Bordetella bronchiseptica

126 portions of the lung tissue samples or nasal swabs with respiratory symptoms, collected from number of pig farms in Henan province, were used to isolate and identify Bordetella bronchiseptica(Bb).According to bacterial culture characteristics, Gram staining, biochemical tests and PCR, 11 strains Bb have been successfully isolated. Three important virulent factors(fha,prn,dnt) for Bb were tested by PCR, most of the PCR results obtained corresponding DNA target bands except that two strains Bb didn’t get prn gene target bands. Erythrocyte agglutination assay showed all kinds of strains were able to agglutinate pig and sheep’s erythrocyte at different degree. Skin necrosis in suckling mice showed all kinds of strains were able to produce skin necrosis to different extent. Virulent strains(HN0610, HN0806, HN0922, HN0827) were confirmed by mouse lethal.